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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 16 — Aug. 8, 2005
  • pp: 6105–6110

Raman imaging of floating cells

Caitriona M. Creely, Giovanni Volpe, Gajendra P. Singh, Marta Soler, and Dmitri V. Petrov  »View Author Affiliations

Optics Express, Vol. 13, Issue 16, pp. 6105-6110 (2005)

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Raman imaging can yield spatially resolved biochemical information from living cells. To date there have been no Raman images published of cells in suspension because of the problem of immobilising them suitably to acquire space-resolved spectra. In this paper in order to overcome this problem the use of holographic optical tweezers is proposed and implemented, and data is shown for spatially resolved Raman spectroscopy of a live cell in suspension.

© 2005 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(230.6120) Optical devices : Spatial light modulators
(300.6330) Spectroscopy : Spectroscopy, inelastic scattering including Raman

ToC Category:
Research Papers

Original Manuscript: June 14, 2005
Revised Manuscript: July 27, 2005
Published: August 8, 2005

Caitriona Creely, Giovanni Volpe, Gajendra Singh, Marta Soler, and Dmitri Petrov, "Raman imaging of floating cells," Opt. Express 13, 6105-6110 (2005)

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