Raman imaging of floating cells

doi:10.1364/OPEX.13.006105

Optics Express

Editor: Michael Duncan
Vol. 13, Iss. 16 — Aug. 8, 2005
pp: 6105–6110

Raman imaging of floating cells

Caitriona Creely, Giovanni Volpe, Gajendra Singh, Marta Soler, and Dmitri Petrov »View Author Affiliations

Optics Express, Vol. 13, Issue 16, pp. 6105-6110 (2005)
http://dx.doi.org/10.1364/OPEX.13.006105

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Abstract
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References (19)
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Abstract

Raman imaging can yield spatially resolved biochemical information from living cells. To date there have been no Raman images published of cells in suspension because of the problem of immobilising them suitably to acquire space-resolved spectra. In this paper in order to overcome this problem the use of holographic optical tweezers is proposed and implemented, and data is shown for spatially resolved Raman spectroscopy of a live cell in suspension.

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(230.6120) Optical devices : Spatial light modulators
(300.6330) Spectroscopy : Spectroscopy, inelastic scattering including Raman

ToC Category:
Research Papers

History
Original Manuscript: June 14, 2005
Revised Manuscript: July 27, 2005
Published: August 8, 2005

Citation
Caitriona Creely, Giovanni Volpe, Gajendra Singh, Marta Soler, and Dmitri Petrov, "Raman imaging of floating cells," Opt. Express 13, 6105-6110 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-16-6105

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References

A. Ashkin, J. M. Dziedzic, and T. Yamane, �??Optical trapping and manipulation of single cells using infrared laser beams,�?? Nature (London) 330, 769�??771 (1987). [CrossRef] [PubMed]
D. G. Grier, �??A revolution in optical manipulation,�?? Nature (London) 424, 810�??816 (2003). [CrossRef] [PubMed]
K. O. Greutich, Micromanipulation by light in biology and medicine: The laser microbeam and optical tweezers (Birkhuser Verlag, Basel, Switzerland, 1999).
M. Lankers, J. Popp, and W. Kiefer, �??Raman and fluorescence spectra of single optically trapped microdroplets in emulsion,�?? Appl. Spec. 48, 1166�??1168 (1994). [CrossRef]
C. Xie, M. A. Dinno, and Y. Li, �??Near-infrared Raman spectroscopy of single optically trapped biological cells,�?? Opt. Lett. 27, 249�??251 (2002). [CrossRef]
K. Ajito and K. Torimitsu, �??Laser trapping and Raman spectroscopy of single cellular organelles in the nanometer range,�?? Lab on a Chip 2, 11�??14 (2002). [CrossRef]
C. M. Creely, G. P. Singh, and D. V. Petrov, �??Dual wavelength optical tweezers for confocal Raman spectroscopy,�?? Opt. Commun. 245, 465�??470 (2004). [CrossRef]
G. P. Singh, C. M. Creely, G. Volpe, H. Grotsch, and D. V. Petrov, �??Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy,�?? Anal. Chem. 77, 2564�??2568 (2005). [CrossRef] [PubMed]
N. Uzunbajakava, A. Lenferink, Y. Kraan, B.Willekens, G. Vrensen, J. Greve, and C. Otto, �??Nonresonant Raman imaging of protein distribution in single human cells,�?? Biopolymers 72, 1�??9 (2003). [CrossRef]
Y. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, �??Raman spectroscopic signature of life in a living yeast cell,�?? J. Ram. Spec. 35, 525�??526 (2004). [CrossRef]
S. C. Grover, R. C. Gauthier, and A. G. Skirtach, �??Analysis of the behaviour of erythrocytes in an optical trapping system,�?? Opt. Express 7, 533�??539 (2000). <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-13-533">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-13-533</a>. [CrossRef] [PubMed]
G. C. Spalding, M. T. Dearing, S. A. Sheets, and D. G. Grier, �??Computer-generated holographic optical tweezer arrays,�?? Rev. Sci. Instrumen. 72, 1810�??1816 (2001). [CrossRef]
H. Melville, G. F. Milne, G. C. Spalding, W. Sibbett, K. Dholakia, and D. C. McGloin, �??Optical trapping of three-dimensional structures using dynamic holograms,�?? Opt. Express 11, 3562�??3567 (2003), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-26-3562">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-26-3562</a>. [CrossRef] [PubMed]
A. Jesacher, S. Frhapter, S. Bernet, and M. Ritsch-Marte, �?? Size selective trapping with optical cogwheel tweezers,�?? Opt. Express 12, 4129�??4135 (2004), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-17-4129">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-17-4129</a>. [CrossRef] [PubMed]
R. L. Eriksen, V. R. Daria, and J. Gl¨uckstad, �??Fully dynamic multiple-beam optical tweezers,�?? Opt. Express 10, 597�??602 (2002), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-14-597">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-14-597</a>. [PubMed]
V. Emiliani, D. Sanvitto, M. Zahid, F. Gerbal, and M. Coppey-Moisan, �??Multi force optical tweezers to generate gradients of forces,�?? Opt. Express 12, 3906�??3910 (2004), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-17-3906">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-17-3906</a>. [CrossRef] [PubMed]
G. Sinclair, P. Jordan, J. Courtial, M. Padgett, J. Cooper and Z. J. Laczik �??Assembly of 3-dimensional structures using programmable holographic optical tweezers,�?? Opt. Express 12, 5475�??5480 (2004), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-22-5475">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-22-5475</a>. [CrossRef] [PubMed]
I. Notingher, I. Bisson, A. E. Bishop, W. L. Randle, J. M. P. Polak, and L. L. Hench, �??In situ spectral monitoring of mRNA translation in embryonic stem cells during differentiation in vitro,�?? Anal. Chem. 76, 3185�??3193 (2004). [CrossRef] [PubMed]
V. Bingelyte, J. Leach, J. Courtial, and M. J. Padgett, �??Optically controlled three-dimensional rotation of microscopic objects,�?? Appl. Phys. Lett. 82, 829�??831 (2003). [CrossRef]

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[1] A. Ashkin, J. M. Dziedzic, and T. Yamane, �??Optical trapping and manipulation of single cells using infrared laser beams,�?? Nature (London) 330, 769�??771 (1987). [CrossRef] [PubMed]

[2] D. G. Grier, �??A revolution in optical manipulation,�?? Nature (London) 424, 810�??816 (2003). [CrossRef] [PubMed]

[3] K. O. Greutich, Micromanipulation by light in biology and medicine: The laser microbeam and optical tweezers (Birkhuser Verlag, Basel, Switzerland, 1999).

[4] M. Lankers, J. Popp, and W. Kiefer, �??Raman and fluorescence spectra of single optically trapped microdroplets in emulsion,�?? Appl. Spec. 48, 1166�??1168 (1994). [CrossRef]

[5] C. Xie, M. A. Dinno, and Y. Li, �??Near-infrared Raman spectroscopy of single optically trapped biological cells,�?? Opt. Lett. 27, 249�??251 (2002). [CrossRef]

[6] K. Ajito and K. Torimitsu, �??Laser trapping and Raman spectroscopy of single cellular organelles in the nanometer range,�?? Lab on a Chip 2, 11�??14 (2002). [CrossRef]

[7] C. M. Creely, G. P. Singh, and D. V. Petrov, �??Dual wavelength optical tweezers for confocal Raman spectroscopy,�?? Opt. Commun. 245, 465�??470 (2004). [CrossRef]

[8] G. P. Singh, C. M. Creely, G. Volpe, H. Grotsch, and D. V. Petrov, �??Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy,�?? Anal. Chem. 77, 2564�??2568 (2005). [CrossRef] [PubMed]

[9] N. Uzunbajakava, A. Lenferink, Y. Kraan, B.Willekens, G. Vrensen, J. Greve, and C. Otto, �??Nonresonant Raman imaging of protein distribution in single human cells,�?? Biopolymers 72, 1�??9 (2003). [CrossRef]

[10] Y. Huang, T. Karashima, M. Yamamoto, T. Ogura, and H. Hamaguchi, �??Raman spectroscopic signature of life in a living yeast cell,�?? J. Ram. Spec. 35, 525�??526 (2004). [CrossRef]

[11] S. C. Grover, R. C. Gauthier, and A. G. Skirtach, �??Analysis of the behaviour of erythrocytes in an optical trapping system,�?? Opt. Express 7, 533�??539 (2000). <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-13-533">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-13-533</a>. [CrossRef] [PubMed]

[12] G. C. Spalding, M. T. Dearing, S. A. Sheets, and D. G. Grier, �??Computer-generated holographic optical tweezer arrays,�?? Rev. Sci. Instrumen. 72, 1810�??1816 (2001). [CrossRef]

[13] H. Melville, G. F. Milne, G. C. Spalding, W. Sibbett, K. Dholakia, and D. C. McGloin, �??Optical trapping of three-dimensional structures using dynamic holograms,�?? Opt. Express 11, 3562�??3567 (2003), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-26-3562">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-26-3562</a>. [CrossRef] [PubMed]

[14] A. Jesacher, S. Frhapter, S. Bernet, and M. Ritsch-Marte, �?? Size selective trapping with optical cogwheel tweezers,�?? Opt. Express 12, 4129�??4135 (2004), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-17-4129">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-17-4129</a>. [CrossRef] [PubMed]

[15] R. L. Eriksen, V. R. Daria, and J. Gl¨uckstad, �??Fully dynamic multiple-beam optical tweezers,�?? Opt. Express 10, 597�??602 (2002), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-14-597">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-14-597</a>. [PubMed]

[16] V. Emiliani, D. Sanvitto, M. Zahid, F. Gerbal, and M. Coppey-Moisan, �??Multi force optical tweezers to generate gradients of forces,�?? Opt. Express 12, 3906�??3910 (2004), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-17-3906">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-17-3906</a>. [CrossRef] [PubMed]

[17] G. Sinclair, P. Jordan, J. Courtial, M. Padgett, J. Cooper and Z. J. Laczik �??Assembly of 3-dimensional structures using programmable holographic optical tweezers,�?? Opt. Express 12, 5475�??5480 (2004), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-22-5475">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-22-5475</a>. [CrossRef] [PubMed]

[18] I. Notingher, I. Bisson, A. E. Bishop, W. L. Randle, J. M. P. Polak, and L. L. Hench, �??In situ spectral monitoring of mRNA translation in embryonic stem cells during differentiation in vitro,�?? Anal. Chem. 76, 3185�??3193 (2004). [CrossRef] [PubMed]

[19] V. Bingelyte, J. Leach, J. Courtial, and M. J. Padgett, �??Optically controlled three-dimensional rotation of microscopic objects,�?? Appl. Phys. Lett. 82, 829�??831 (2003). [CrossRef]

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Raman imaging of floating cells