OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 16 — Aug. 8, 2005
  • pp: 6275–6285

Time-resolved fluorescence imaging of solvent interactions in microfluidic devices

Richard K. P. Benninger, Oliver Hofmann, James McGinty, Jose Requejo-Isidro, Ian Munro, Mark A. A. Neil, Andrew J. deMello, and Paul M. W. French  »View Author Affiliations


Optics Express, Vol. 13, Issue 16, pp. 6275-6285 (2005)
http://dx.doi.org/10.1364/OPEX.13.006275


View Full Text Article

Enhanced HTML    Acrobat PDF (3345 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present the application of wide-field time-resolved fluorescence imaging methods for the study of solvent interactions and mixing in microfluidic devices. Time-resolved imaging of fluorescence polarization anisotropy allows us to image the local viscosity of fluorescein in three dimensions in order to directly monitor solvent mixing within a microfluidic channel. This provides a viscosity image acquisition time of the order of minutes, and has been applied to a steady-state laminar flow configuration. To image dynamic fluid mixing in real-time, we demonstrate high-speed fluorescence lifetime imaging at 12.3 Hz applied to DASPI, which directly exhibits a solvent viscosity-dependant fluorescence lifetime. These two methods facilitate a high degree of quantification of microfluidic flow in 3-D and/or at high speed, providing a tool for studying fluid dynamics and for developing enhanced microfluidic assays.

© 2005 Optical Society of America

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(320.5390) Ultrafast optics : Picosecond phenomena

ToC Category:
Research Papers

History
Original Manuscript: April 6, 2005
Revised Manuscript: August 1, 2005
Published: August 8, 2005

Citation
Richard Benninger, Oliver Hofmann, James McGinty, Jose Requejo-Isidro, Ian Munro, Mark Neil, Andrew deMello, and Paul French, "Time-resolved fluorescence imaging of solvent interactions in microfluidic devices," Opt. Express 13, 6275-6285 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-16-6275


Sort:  Journal  |  Reset  

References

  1. D. R. Reyes, D. Iossifidis, P. A. Auroux and A. Manz, "Micro total analysis systems. 1. Introduction, theory, and technology," Anal. Chem. 74, 2623-2636 (2002). [CrossRef] [PubMed]
  2. P. A. Auroux, D. Iossifidis, D. R. Reyes and A. Manz, "Micro total analysis systems. 2. Analytical standard operations and applications," Anal. Chem. 74, 2637-2652 (2002). [CrossRef] [PubMed]
  3. T. Vilkner, D. Janasek and A. Manz, "Micro total analysis systems. Recent developments," Anal. Chem. 76, 3373-3385 (2004). [CrossRef] [PubMed]
  4. E. Verpoorte, "Chip vision - optics for microchips," Lab Chip 3, 42N-52N (2003).
  5. A. E. Kamholz, B. H. Weigl, B. A. Finlayson and P. Yager, "Quantitative analysis of molecular interaction in a microfluidic channel: The T-sensor," Anal. Chem. 71, 5340-5347 (1999). [CrossRef] [PubMed]
  6. A. Hatch, A. E. Kamholz, K. R. Hawkins, M. S. Munson, E. A. Schilling, B. H. Weigl and P. Yager, "A rapid diffusion immunoassay in a T-sensor," Nat. Biotechnol. 19, 461-465 (2001). [CrossRef] [PubMed]
  7. D. Ross, M. Gaitan and L. E. Locascio, "Temperature measurement in microfluidic systems using a temperature-dependent fluorescent dye," Anal. Chem. 73, 4117-4123 (2001). [CrossRef] [PubMed]
  8. R. P. Hertzberg and A. J. Pope, "High-throughput screening: new technology for the 21st century," Curr. Opin. Chem. Biol. 4, 445-451 (2000). [CrossRef] [PubMed]
  9. K. Suhling, J. Siegel, P. M. P. Lanigan, S. Leveque-Fort, S. E. D. Webb, D. Phillips, D. M. Davis and P. M. W. French, "Time-resolved fluorescence anisotropy imaging applied to live cells," Opt. Lett. 29, 584-586 (2004). [CrossRef] [PubMed]
  10. A. H. A. Clayton, Q. S. Hanley, D. J. Arndt-Jovin, V. Subramaniam and T. M. Jovin, "Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM)," Biophys. J. 83, 1631-1649 (2002). [CrossRef] [PubMed]
  11. G. H. Patterson and D. W. Piston, "Photobleaching in two-photon excitation microscopy," Biophys. J. 78, 2159-2162 (2000). [CrossRef] [PubMed]
  12. J. Requejo-Isidro, J. McGinty, I. Munro, D. S. Elson, N. P. Galletly, M. J. Lever, M. A. A. Neil, G. W. H. Stamp, P. M. W. French, P. A. Kellett, J. D. Hares and A. K. L. Dymoke-Bradshaw, "High-speed widefield time-gated endoscopic fluorescence- lifetime imaging," Opt. Lett. 29, 2249-2251 (2004). [CrossRef] [PubMed]
  13. R. K. P. Benninger, B. Onfelt, M. A. A. Neil, D. M. Davis and P. M. W. French, "Fluorescence imaging of two-photon linear dichroism: Cholesterol depletion disrupts molecular orientation in cell membranes," Biophys. J. 88, 609-622 (2005). [CrossRef]
  14. J. Siegel, K. Suhling, S. Lévêque-Fort, S. E. D. Webb, D. M. Davis, D. Phillips, Y. Sabharwal and P. M. W. French, "Wide-field time-resolved fluorescence anisotropy imaging (TR- FAIM): Imaging the rotational mobility of a fluorophore," Rev. Sci. Instrum. 74, 182-192 (2003). [CrossRef]
  15. M. Tramier, K. Kemnitz, C. Durieux, J. Coppey, P. Denjean, R. B. Pansu and M. Coppey-moisan, "Restrained torsional dynamics of nuclear DNA in living proliferative mammalian cells," Biophys. J. 78, 2614-2627 (2000). [CrossRef] [PubMed]
  16. D. Axelrod, "Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization," Biophys. J. 26, 557-573 (1979). [CrossRef] [PubMed]
  17. C. Z. Wan and C. K. Johnson, "Time-Resolved Anisotropic 2-Photon Spectroscopy," Chem. Phys. 179, 513-531 (1994). [CrossRef]
  18. A. V. Agronskaia, L. Tertoolen and H. C. Gerritsen, "High frame rate fluorescence lifetime imaging," J. Phys. D. 36, 1655-1662 (2003). [CrossRef]
  19. K. Dowling, M. J. Dayel, S. C. W. Hyde, P. M. W. French, M. J. Lever, J. D. Hares and A. K. L. Dymoke-Bradshaw, "High resolution time-domain fluorescence lifetime imaging for biomedical applications," J. Mod. Opt. 46, 199-209 (1999).
  20. J. R. Lakowicz, "Principles of Fluorescence Spectroscopy 2nd edition" (Kluwer Academic/Plenum Publishers: New York,1999).
  21. J. R. Taylor, M. C. Adams and W. Sibbett, "Investigation of viscosity dependent fluorescence lifetime using a synchronously operated picosecond streak camera," Appl. Phys. 21, 13-17 (1980). [CrossRef]
  22. M. J. Cole, J. Siegel, S. E. D. Webb, R. Jones, K. Dowling, P. M. W. French, M. J. Lever, L. O. D. Sucharov, M. A. A. Neil, R. Juskaitis and T. Wilson, "Whole-field optically sectioned fluorescence lifetime imaging," Opt. Lett. 25, 1361-1363 (2000). [CrossRef]
  23. D. Grant, E. Auksorius, D. N. Schimpf, P. M. P. Lanigan, P. A. A. De Beule, J. McGinty, D. S. Elson, C. Dunsby, J. Requejo-Isidro, I. Munro, N. Galletly, G. W. H. Stamp, P. Courtney, M. A. A. Neil and P. M. W. French, "An Electronically Tuneable Ultrafast Laser Source applied to Fluorescence Imaging including Wide-Field Optically-Sectioned Fluoescence Lifetime Imaging using a Nipkow Disk Microscope" presented at Focus on Microscopy, Jena, Germany, 20-23 March, 2005.
  24. K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French and J. D. Hares, "2-D fluorescence lifetime imaging using a time-gated image intensifier," Opt. Commun. 135, 27-31 (1997). [CrossRef]
  25. D. S. Elson, I. Munro, J. Requejo-Isidro, J. McGinty, C. Dunsby, N. Galletly, G. W. Stamp, M. A. A. Neil, M. J. Lever, P. A. Kellett, A. Dymoke-Bradshaw, J. Hares and P. M. W. French, "Real-time time-domain fluorescence lifetime imaging including single-shot acquisition with a segmented optical image intensifier," New J. Phys. 6, art. no.-180 (2004). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Multimedia

Multimedia FilesRecommended Software
» Media 1: AVI (3420 KB)     
» Media 2: AVI (3419 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited