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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 16 — Aug. 8, 2005
  • pp: 6275–6285

Time-resolved fluorescence imaging of solvent interactions in microfluidic devices

Richard K. P. Benninger, Oliver Hofmann, James McGinty, Jose Requejo-Isidro, Ian Munro, Mark A. A. Neil, Andrew J. deMello, and Paul M. W. French  »View Author Affiliations

Optics Express, Vol. 13, Issue 16, pp. 6275-6285 (2005)

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We present the application of wide-field time-resolved fluorescence imaging methods for the study of solvent interactions and mixing in microfluidic devices. Time-resolved imaging of fluorescence polarization anisotropy allows us to image the local viscosity of fluorescein in three dimensions in order to directly monitor solvent mixing within a microfluidic channel. This provides a viscosity image acquisition time of the order of minutes, and has been applied to a steady-state laminar flow configuration. To image dynamic fluid mixing in real-time, we demonstrate high-speed fluorescence lifetime imaging at 12.3 Hz applied to DASPI, which directly exhibits a solvent viscosity-dependant fluorescence lifetime. These two methods facilitate a high degree of quantification of microfluidic flow in 3-D and/or at high speed, providing a tool for studying fluid dynamics and for developing enhanced microfluidic assays.

© 2005 Optical Society of America

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(320.5390) Ultrafast optics : Picosecond phenomena

ToC Category:
Research Papers

Original Manuscript: April 6, 2005
Revised Manuscript: August 1, 2005
Published: August 8, 2005

Richard Benninger, Oliver Hofmann, James McGinty, Jose Requejo-Isidro, Ian Munro, Mark Neil, Andrew deMello, and Paul French, "Time-resolved fluorescence imaging of solvent interactions in microfluidic devices," Opt. Express 13, 6275-6285 (2005)

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