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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 16 — Aug. 8, 2005
  • pp: 6286–6295

In vivo anterior segment imaging in the rat eye with high speed white light full-field optical coherence tomography

Kate Grieve, Arnaud Dubois, Manuel Simonutti, Michel Paques, José Sahel, Jean-François Le Gargasson, and Claude Boccara  »View Author Affiliations


Optics Express, Vol. 13, Issue 16, pp. 6286-6295 (2005)
http://dx.doi.org/10.1364/OPEX.13.006286


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Abstract

We present a new high speed full-field optical coherence tomography (OCT) instrument, the first full-field OCT system that is capable of in vivo ocular imaging. An isotropic resolution of ~ 1 μm is achieved thanks to the use of a xenon arc lamp source and relatively high numerical aperture microscope objectives in a Linnik-type interferometer. Full-field illumination allows the capture of two-dimensional en face images in parallel, using a fast CMOS camera as detector array. Each en face image is acquired in a 4 ms period, at a maximum repetition rate of 250 Hz. Detection sensitivity per en face image is 71 dB. Higher sensitivity can be achieved by image correlation and averaging, although frame rate is reduced. We present the first preliminary results of in vivo imaging in the anterior segment of the rat eye, which reveal some cellular features in the corneal layers.

© 2005 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Research Papers

History
Original Manuscript: May 20, 2005
Revised Manuscript: August 1, 2005
Published: August 8, 2005

Citation
Kate Grieve, Arnaud Dubois, Manuel Simonutti, Michel Paques, José Sahel, Jean-François Le Gargasson, and Claude Boccara, "In vivo anterior segment imaging in the rat eye with high speed white light full-field optical coherence tomography," Opt. Express 13, 6286-6295 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-16-6286


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