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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 17 — Aug. 22, 2005
  • pp: 6597–6614

Magnetomotive contrast for in vivo optical coherence tomography

Amy L. Oldenburg, Farah Jean-Jacques Toublan, Kenneth S. Suslick, Alexander Wei, and Stephen A. Boppart  »View Author Affiliations

Optics Express, Vol. 13, Issue 17, pp. 6597-6614 (2005)

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Molecularly-specific contrast can greatly enhance the biomedical utility of optical coherence tomography (OCT). We describe a contrast mechanism, magnetomotive OCT (MMOCT), where a modulated magnetic field induces motion of magnetic nanoparticles. The motion of the nanoparticles modifies the amplitude of the OCT interferogram. High specificity is achieved by subtracting the background fluctuations of the specimen, and sensitivity to 220 μg/g magnetite nanoparticles is demonstrated. Optically and mechanically correct tissue phantoms elucidate the relationships between imaging contrast and nanoparticle concentration, imaging depth, tissue optical scattering, and magnetic field strength. MMOCT is demonstrated in a living Xenopus laevis tadpole where the results were consistent with corresponding histology.

© 2005 Optical Society of America

OCIS Codes
(160.3820) Materials : Magneto-optical materials
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(290.5850) Scattering : Scattering, particles

ToC Category:
Research Papers

Original Manuscript: July 6, 2005
Revised Manuscript: August 15, 2005
Published: August 22, 2005

Amy Oldenburg, Farah Toublan, Kenneth Suslick, Alexander Wei, and Stephen Boppart, "Magnetomotive contrast for in vivo optical coherence tomography," Opt. Express 13, 6597-6614 (2005)

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  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178 (1991). [CrossRef] [PubMed]
  2. Y. Jiang, I. Tornov, Y. Wang, and Z. Chen, "Second-harmonic optical coherence tomography," Opt. Lett. 29, 1090 (2004). [CrossRef] [PubMed]
  3. D. L. Marks and S. A. Boppart, "Nonlinear interferometric vibrational imaging," Phys. Rev. Lett. 92, 123905 (2004). [CrossRef] [PubMed]
  4. C. Vinegoni, J. S. Bredfeldt, D. L. Marks, and S. A. Boppart, "Nonlinear optical contrast enhancement for optical coherence tomography," Opt. Express 12, 331 (2004), <a href="http://www.opticsexpress.org/abstract.cfm?"URI=OPEX-12-2-331">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-2-331</a> [CrossRef] [PubMed]
  5. J. S. Bredfeldt, C. Vinegoni, D. L. Marks, and S. A. Boppart, "Molecularly sensitive optical coherence tomography," Opt. Lett. 30, 495 (2005). [CrossRef] [PubMed]
  6. C. Loo, A. Lin, L. Hirsch, M.-H. Lee, J. Barton, N. Halas, J. West, and R. Drezek, "Nanoshell-enabled photonics-based imaging and therapy of cancer," Technol. Cancer Res. Treat. 3, 33 (2004). [PubMed]
  7. T.-M. Lee, A. L. Oldenburg, S. Sitafalwalla, D. L. Marks, W. Luo, F. Jean-Jacques Toublan, K. S. Suslick, and S. A. Boppart, "Engineered microsphere contrast agents for optical coherence tomography," Opt. Lett. 28, 1546 (2003). [CrossRef] [PubMed]
  8. U. Morgner, W. Drexler, F. X. Kartner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, "Spectroscopic optical coherence tomography," Opt. Lett. 25, 111 (2000). [CrossRef]
  9. D. J. Faber, E. G. Mik, M. C. G. Aalders, and T. G. van Leeuwen, "Light absorption of (oxy-)hemoglobin assessed by spectroscopic optical coherence tomography," Opt. Lett. 28, 1436 (2003). [CrossRef] [PubMed]
  10. C. Xu, J. Ye, D. L. Marks, and S. A. Boppart, "Near-infrared dyes as contrast-enhancing agents for spectroscopic optical coherence tomography," Opt. Lett. 29, 1647 (2004). [CrossRef] [PubMed]
  11. C. Yang, L. E. L. McGuckin, J. D. Simon, M. A. Choma, B. E. Applegate, and J. A. Izatt, "Spectral triangulation molecular contrast optical coherence tomography with indocyanine green as the contrast agent," Opt. Lett. 29, 2016 (2004). [CrossRef] [PubMed]
  12. K. D. Rao, M. A. Choma, S. Yazdanfar, A. M. Rollins, and J. A. Izatt, "Molecular contrast in optical coherence tomography by use of a pump-probe technique," Opt. Lett. 28, 340 (2003). [CrossRef] [PubMed]
  13. C. Yang, M. A. Choma, L. E. Lamb, J. D. Simon, and J. A. Izatt, "Protein-based molecular contrast optical coherence tomography with phytochrome as the contrast agent," Opt. Lett. 29, 1396 (2004). [CrossRef] [PubMed]
  14. H. Watarai and M. Namba, "Capillary magnetophoresis of human blood cells and their magnetophoretic trapping in a flow system," J. Chromatogr. A 961, 3 (2002). [CrossRef] [PubMed]
  15. S. Palmacci and L. Josephson, "Synthesis of polysaccharaide covered superparamagnetic oxide colloids," United States Patent #5,262,176 (1993).
  16. M. G. Harisinghani, J. Barentsz, P. F. Hahn, W. M. Deserno, S. Tabatabaei, C. Hulsbergen van de Kaa, J. de la Rosette, and R. Weissleder, "Noninvasive detection of clinically occult lymph-node metastases in prostate cancer," N. Engl. J. Med. 348, 2491 (2003). [CrossRef] [PubMed]
  17. A. S. Arbab, G. T. Yocum, L. B. Wilson, A. Parwana, E. K. Jordan, H. Kalish, and J. A. Frank, "Comparison of transfection agents in forming complexes with ferumoxides, cell labeling efficiency, and cellular viability," Mol. Imaging 3, 24 (2004). [CrossRef] [PubMed]
  18. A. L. Oldenburg, J. R. Gunther, and S. A. Boppart, "Imaging magnetically labeled cells with magnetomotive optical coherence tomography," Opt. Lett. 30, 747 (2005). [CrossRef] [PubMed]
  19. J. W. Goodman, Statistical Optics (John Wiley & Sons, 1985).
  20. U. Hafeli, W. Schutt, J. Teller, and M. Zborowski, Scientific and Clinical Applications of Magnetic Carriers (Plenum Press, 1997).
  21. J. M. Schmitt, "Restoration of optical coherence images of living tissue using the CLEAN algorithm," J. Biomed. Opt. 3, 66 (1998). [CrossRef]
  22. M. Pircher, E. Gotzinger, R. Leitgeb, A. F. Fercher, and C. K. Hitzenberger, "Speckle reduction in optical coherence tomography by frequency compounding," J. Biomed. Opt. 8, 565 (2003). [CrossRef] [PubMed]
  23. G. Yao, L. V. Wang, "Signal dependence and noise source in ultrasound-modulated optical tomography," Appl. Opt. 43, 1320 (2004). [CrossRef] [PubMed]
  24. S. Hamaguchi, I. Tohnai, A. Ito, K. Mitsudo, T. Shigetomi, M. Ito, H. Honda, T. Kobayashi, and M. Ueda, �??Selective hyperthermia using magnetoliposomes to target cervical lymph node metastasis in a rabbit tongue tumor model,�?? Cancer Sci. 94, 834 (2003). [CrossRef] [PubMed]
  25. A. L. Oldenburg, J. R. Gunther, F. Jean-Jacques Toublan, D. L. Marks, K. S. Suslick, and S. A. Boppart, "Selective OCT imaging of cells using magnetically-modulated optical contrast agents," in Proceedings of the Conference on Lasers and Electro-Optics, pp. 405-406 (2003).
  26. M. Wojtkowski, V. J. Srinvasan, T. H. Ko, J. G. Fujimoto, A. Kowalczyk, and J. S. Duker, "Ultrahigh-resolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation," Opt. Express 12, 2404 (2004), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404</a> [CrossRef] [PubMed]
  27. T. L Troy and S. N. Thennadil, "Optical properties of human skin in the near infrared wavelength range of 1000 to 2200 nm," J. Biomed. Opt. 6, 167 (2001). [CrossRef] [PubMed]
  28. J. Schmitt, "OCT elastography: imaging microscopic deformation and strain of tissue," Opt. Express 3, 199 (1998), <a href=" http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-6-199">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-6-199</a> [CrossRef] [PubMed]
  29. D. C. Cannatella and R. O. De Sa, "Xenopus laevis as a model organism," Syst. Biol. 42, 476 (1993). [CrossRef]
  30. G. Bernardini, M. Prati, E. Boneti, and G. Scari, Atlas of Xenopus Development (Springer, 1999).

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