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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 18 — Sep. 5, 2005
  • pp: 6756–6771

Practical reconstruction method for bioluminescence tomography

Wenxiang Cong, Ge Wang, Durairaj Kumar, Yi Liu, Ming Jiang, Lihong Wang, Eric Hoffman, Geoffrey McLennan, Paul McCray, Joseph Zabner, and Alexander Cong  »View Author Affiliations

Optics Express, Vol. 13, Issue 18, pp. 6756-6771 (2005)

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Bioluminescence tomography (BLT) is used to localize and quantify bioluminescent sources in a small living animal. By advancing bioluminescent imaging to a tomographic framework, it helps to diagnose diseases, monitor therapies and facilitate drug development. In this paper, we establish a direct linear relationship between measured surface photon density and an unknown bioluminescence source distribution by using a finite-element method based on the diffusion approximation to the photon propagation in biological tissue. We develop a novel reconstruction algorithm to recover the source distribution. This algorithm incorporates a priori knowledge to define the permissible source region in order to enhance numerical stability and efficiency. Simulations with a numerical mouse chest phantom demonstrate the feasibility of the proposed BLT algorithm and reveal its performance in terms of source location, density, and robustness against noise. Lastly, BLT experiments are performed to identify the location and power of two light sources in a physical mouse chest phantom.

© 2005 Optical Society of America

OCIS Codes
(110.6960) Imaging systems : Tomography
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Research Papers

Original Manuscript: July 11, 2005
Revised Manuscript: August 16, 2005
Published: September 5, 2005

Wenxiang Cong, Ge Wang, Durairaj Kumar, Yi Liu, Ming Jiang, Lihong Wang, Eric Hoffman, Geoffrey McLennan, Paul McCray, Joseph Zabner, and Alexander Cong, "Practical reconstruction method for bioluminescence tomography," Opt. Express 13, 6756-6771 (2005)

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