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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 19 — Sep. 19, 2005
  • pp: 7466–7482

Time-resolved photon migration in bi-layered tissue models

Karthik Vishwanath and Mary-Ann Mycek  »View Author Affiliations

Optics Express, Vol. 13, Issue 19, pp. 7466-7482 (2005)

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In this article, we describe a novel Monte Carlo code for time-integrated and time-resolved photon migration simulations of excitation and fluorescent light propagation (with reabsorption) in bi-layered models of biological tissues. The code was experimentally validated using bi-layered, tissue-simulating phantoms and the agreement between simulations and experiment was better than 3%. We demonstrate the utility of the code for quantitative clinical optical diagnostics in epithelial tissues by examining design characteristics for clinically compatible waveguides with arbitrarily complex source-detector configurations. Results for human colonic tissues included a quantitative comparison of simulation predictions with time-resolved fluorescence data measured in vivo and spatio-temporal visualizations of photon migration characteristics in tissue models in both two- and three-dimensions for source-detector configurations, including variable waveguide spacing, numerical aperture, and diameter. These results were then extended from surface point spectroscopy to imaging modalities for both time-gated (fluorescence lifetime) and steady-state (fluorescence intensity) experimental conditions. To illustrate the flexibility of this computational approach, time-domain results were extended to simulate predictions for frequency-domain instrumentation. This work is the first demonstration and validation of a time-domain, multi-wavelength photon transport model with these capabilities in layered turbid-media.

© 2005 Optical Society of America

OCIS Codes
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Research Papers

Original Manuscript: July 19, 2005
Revised Manuscript: September 2, 2005
Published: September 19, 2005

Karthik Vishwanath and Mary-Ann Mycek, "Time-resolved photon migration in bi-layered tissue models," Opt. Express 13, 7466-7482 (2005)

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