OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 2 — Jan. 24, 2005
  • pp: 382–388

Depth-resolved fluorescence spectroscopy of normal and dysplastic cervical tissue

Yicong Wu, Peng Xi, Jianan Y. Qu, Tak-Hong Cheung, and Mei-Yung Yu  »View Author Affiliations

Optics Express, Vol. 13, Issue 2, pp. 382-388 (2005)

View Full Text Article

Enhanced HTML    Acrobat PDF (540 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A portable confocal system with the excitations at 355nm and 457nm was instrumented to investigate the depth-resolved fluorescence of cervical tissue. The study focused on extracting biochemical and morphological information carried in the depth-resolved signals measured from the normal squamous epithelial tissue and squamous intraepithelial lesions. Strong keratin fluorescence with the spectral characteristics similar to collagen were observed from the topmost keratinizing layer of all tissue samples. It was found that NADH and FAD fluorescence measured from the underlying non-keratinizing epithelial layer were strongly correlated to the tissue pathology. This study demonstrates that the depth-resolved fluorescence spectroscopy can potentially provide more accurate diagnostic information for determining tissue pathology.

© 2005 Optical Society of America

OCIS Codes
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Research Papers

Original Manuscript: November 22, 2004
Revised Manuscript: January 3, 2005
Published: January 24, 2005

Yicong Wu, Peng Xi, Jianan Qu, Tak-Hong Cheung, and Mei-Yung Yu, "Depth-resolved fluorescence spectroscopy of normal and dysplastic cervical tissue," Opt. Express 13, 382-388 (2005)

Sort:  Journal  |  Reset  


  1. N. Ramanujam, M. F. Mitchell, A. Mahadevan, S. Warren, S. Thomsen, E. Silva and R. Richards-Kortum, �??In vivo diagnosis of cervical intraepithelial neoplasia using 377-nm-excited laser-induced fluorescence,�?? Proc. Natl. Acad. Sci. USA. 91, 10193-10197 (1994) [CrossRef] [PubMed]
  2. S. K. Chang, M. Follen, A. Malpica, U. Utzinger, G. Staerkel, D. Cox, E. N. Atkinson, C. MacAulay and R. Richards-Kortum, �??Optimal excitation wavelengths for discrimination of cervical neoplasia,�?? IEEE Trans. Biomed. Eng. 49, 1102-1110 (2002) [CrossRef] [PubMed]
  3. R. Drezek, C. Brookner, I. Pavlova, I. Boiko, A. Malpica, R. Lotan, M. Follen and R. Richards-Kortum, �??Autofluoresecence microscopy of fresh cervical-tissue sections reveals alterations in tissue biochemistry with dysplasia,�?? Photochem. Photobiol. 73, 636-641 (2001) [CrossRef] [PubMed]
  4. I. Pavlova, K. Sokolov K, R. Drezek, A. Malpica, M. Follen and R. Richards-Kortum, "Microanatomical and biochemical origins of normal and precancerous cervical autofluorescence using laser-scanning fluorescence confocal microscopy," Photochem. Photobiol. 77, 550-555 (2003) [CrossRef] [PubMed]
  5. Y. Wu, P. Xi, J. Y. Qu, T. Cheung and M. Yu, �??Depth-resolved fluorescence spectroscopy reveals layered structure of tissue,�?? Opt. Express 12, 3218-3223 (2004) <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-14-3218">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-14-3218</a> [CrossRef] [PubMed]
  6. M. G. Muller, T. A. Valdez, I. Georgakoudi, V. Backman, C. Fuentes, S. Kabani, N. Laver, Z. Wang, C. W. Boone, R R. Dasari, S. M. Shapshay, M. S. Feld, �??Spectroscopic detection and evaluation of morphologic and biochemical changes in early human oral carcinoma,�?? Cancer 97, 1681-169 (2003) [CrossRef] [PubMed]
  7. B Chance, B. Schoener, R. Oshino, F. Itshak, and Y. Nakase, �??Oxidation-reduction ratio studies of mitochondria in freeze-trapped samples,�?? J. Biol. Chem. 254, 4764-4771 (1979) [PubMed]
  8. G. M. Palmer, C. L. Marshek, K. M. Vrotsos and N. Ramanujam, "Optimal methods for fluorescence and diffuse reflectance measurements of tissue biopsy samples," Lasers Surg. Med. 30, 191-200 (2002) [CrossRef] [PubMed]
  9. T. Collier, D. Arifler, A. Malpica, M. Follen and R. Richards-Kortum, �??Determination of Epithelial tissue scattering coefficient using confocal microscopy,�?? IEEE. J. Sel. Top. Quantum Electron. 9, 307-313(2003) [CrossRef]
  10. C. Carrilho, M. Alberto, L. Buane and L. David, �??Keratins 8, 10, 13, and 17 are useful markers in the diagnosis of human cervix carcinomas,�?? Hum. Pathol. 35, 546-551(2004) [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

Supplementary Material

» Media 1: MOV (210 KB)     
» Media 2: MOV (250 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited