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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 20 — Oct. 3, 2005
  • pp: 8231–8242

Optical biopsy of fixed human skin with backward-collected optical harmonics signals

Shih-Peng Tai, Tsung-Han Tsai, Wen-Jeng Lee, Dar-Bin Shieh, Yi-Hua Liao, Hsin-Yi Huang, Kenneth Y.-J. Zhang, Hsiang-Lin Liu, and Chi-Kuang Sun  »View Author Affiliations

Optics Express, Vol. 13, Issue 20, pp. 8231-8242 (2005)

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Harmonics-based optical microscopy has been widely applied in biomedical researches due to its noninvasiveness to the studied biomaterials. Due to momentum conservation consideration, most previous studies collect harmonics generation signals in a forward geometry, especially for third harmonic generation signals. However, the adopted forward transmission type geometry is not feasible for future clinical diagnosis. In this paper, first virtual biopsy based on backward propagating optical higher harmonics, combining second harmonic and third harmonic, is demonstrated in the fixed human skin specimens. In our study, third harmonic generation can provide morphologic information including the distribution of basal cells while second harmonic generation can provide distribution of collagen fibers in dermis. Therefore, this technique is ideal for future noninvasive in vivo skin disease examination without dye.

© 2005 Optical Society of America

OCIS Codes
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

ToC Category:
Research Papers

Original Manuscript: August 22, 2005
Revised Manuscript: September 24, 2005
Published: October 3, 2005

Shih-Peng Tai, Tsung-Han Tsai, Wen-Jeng Lee, Dar-Bin Shieh, Yi-Hua Liao, Hsin-Yi Huang, Kenneth Zhang, Hsiang-Lin Liu, and Chi-Kuang Sun, "Optical biopsy of fixed human skin with backward-collected optical harmonics signals," Opt. Express 13, 8231-8242 (2005)

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