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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 22 — Oct. 31, 2005
  • pp: 8693–8698

High-resolution quantitative phase-contrast microscopy by digital holography

Christopher J. Mann, Lingfeng Yu, Chun-Min Lo, and Myung K. Kim  »View Author Affiliations


Optics Express, Vol. 13, Issue 22, pp. 8693-8698 (2005)
http://dx.doi.org/10.1364/OPEX.13.008693


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Abstract

Techniques of digital holography are improved in order to obtain high-resolution, high-fidelity images of quantitative phase-contrast microscopy. In particular, the angular spectrum method of calculating holographic optical field is seen to have significant advantages including tight control of spurious noise components. Holographic phase images are obtained with 0.5 μm diffraction-limited lateral resolution and largely immune from the coherent noise common in other holographic techniques. The phase profile is accurate to about 30 nm of optical thickness. Images of SKOV-3 ovarian cancer cells display intracellular and intranuclear organelles with clarity and quantitative accuracy.

© 2005 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(090.2880) Holography : Holographic interferometry
(180.3170) Microscopy : Interference microscopy

ToC Category:
Research Papers

History
Original Manuscript: July 25, 2005
Revised Manuscript: September 9, 2005
Published: October 31, 2005

Citation
Christopher Mann, Lingfeng Yu, Chun-Min Lo, and Myung Kim, "High-resolution quantitative phase-contrast microscopy by digital holography," Opt. Express 13, 8693-8698 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-22-8693


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References

  1. P. Torok and F.J. Kao, Optical imaging and microscopy (Springer-Verlag, 2003).
  2. A. Barty, K. A. Nugent, D. Paganin and A. Roberts, �??Quantitative optical phase microscopy,�?? Opt. Lett. 23, 817-9 (1998). [CrossRef]
  3. G. Popescu, L. P. Delflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari and M. S. Feld, �??Fourier phase microscopy for investigation of biological structures and dynamics,�?? Opt. Lett. 29, 2503-5 (2004). [CrossRef] [PubMed]
  4. C. G. Rylander, D. Dave, T. Akkin, T. E. Milner, K. R. Diller and A. J. Welch, �??Quantitative phase-contrast imaging of cells with phase-sensitive optical coherence microscopy�??. Opt. Lett. 29, 1509-11 (2004). [CrossRef] [PubMed]
  5. U. Schnars and W.P. Jueptner, Digital Holography (Springer-Verlag, 2005).
  6. E. Cuche, F. Bevilacqua, and C. Depeursinge, �??Digital holography for quantitative phase-contrast imaging,�?? Opt. Lett. 24, 291-3 (1999). [CrossRef]
  7. L. Xu, X. Peng, J. Miao, and A.K. Asundi, �??Studies of digital microscopic holography with applications to microstructure testing�??, Appl. Opt. 40, 5046-51 (2001). [CrossRef]
  8. I. Yamaguchi, J. Kato, S. Ohta, and J. Mizuno, �??Image formation in phase-shifting digital holography and applications to microscopy,�?? Appl. Opt. 40, 6177-86 (2001). [CrossRef]
  9. Zhang, T. and I. Yamaguchi, �??Three-dimensional microscopy with phase-shifting digital holography,�?? Opt. Lett. 23, 1221 (1998). [CrossRef]
  10. P. Ferraro, S. De Nicola, A. Finizio, G. Coppola, S. Grilli, C. Magro, and G. Pierattini, �??Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging,�?? Appl. Opt. 42, 1938-46 (2003). [CrossRef] [PubMed]
  11. M.K. Kim, �??Tomographic three-dimensional imaging of a biological specimen using wavelength-scanning digital interference holography,�?? Opt. Express 7, 305-10 (2000), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-9-305">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-9-305</a> [CrossRef] [PubMed]
  12. J. Gass, A. Dakoff, and M.K. Kim, �??Phase imaging without 2�? ambiguity by multiple-wavelength digital holography,�?? Opt. Lett. 28, 1141-3 (2003). [CrossRef] [PubMed]
  13. Marquet, B. Rappaz, P.J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, �??Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwevelength axial accuracy�??, Opt. Lett. 30, 468-70 (2005). [CrossRef] [PubMed]
  14. J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill, 1996).
  15. Lingfeng Yu and Myung K Kim, �??Wavelength-scanning digital interference holography for tomographic 3D imaging using the angular spectrum method,�?? Opt. Lett. 30, 2092 (2005). [CrossRef] [PubMed]
  16. T. M. Kreis, M. Adams and W. P. O. Jueptner, �??Methods of digital holography: a comparison,�?? Proc. SPIE 3098, 224-33 (1997). [CrossRef]

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