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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 22 — Oct. 31, 2005
  • pp: 8693–8698

High-resolution quantitative phase-contrast microscopy by digital holography

Christopher J. Mann, Lingfeng Yu, Chun-Min Lo, and Myung K. Kim  »View Author Affiliations

Optics Express, Vol. 13, Issue 22, pp. 8693-8698 (2005)

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Techniques of digital holography are improved in order to obtain high-resolution, high-fidelity images of quantitative phase-contrast microscopy. In particular, the angular spectrum method of calculating holographic optical field is seen to have significant advantages including tight control of spurious noise components. Holographic phase images are obtained with 0.5 μm diffraction-limited lateral resolution and largely immune from the coherent noise common in other holographic techniques. The phase profile is accurate to about 30 nm of optical thickness. Images of SKOV-3 ovarian cancer cells display intracellular and intranuclear organelles with clarity and quantitative accuracy.

© 2005 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(090.2880) Holography : Holographic interferometry
(180.3170) Microscopy : Interference microscopy

ToC Category:
Research Papers

Original Manuscript: July 25, 2005
Revised Manuscript: September 9, 2005
Published: October 31, 2005

Christopher Mann, Lingfeng Yu, Chun-Min Lo, and Myung Kim, "High-resolution quantitative phase-contrast microscopy by digital holography," Opt. Express 13, 8693-8698 (2005)

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