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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 24 — Nov. 28, 2005
  • pp: 9847–9857

A finite-element-based reconstruction method for 3D fluorescence tomography

Alexander X. Cong and Ge Wang  »View Author Affiliations


Optics Express, Vol. 13, Issue 24, pp. 9847-9857 (2005)
http://dx.doi.org/10.1364/OPEX.13.009847


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Abstract

In this paper, we propose a dual-excitation-mode methodology for three-dimensional (3D) fluorescence molecular tomography (FMT). For this modality, an effective reconstruction algorithm is developed to reconstruct fluorescent yield and lifetime using finite element techniques. In the steady state mode, a direct linear relationship is established between measured optical data on the body surface of a small animal and the unknown fluorescent yield inside the animal, and the reconstruction of fluorescent yield is formulated as a linear least square minimization problem. In the frequency domain mode, based on localization results of the fluorescent probe obtained using the first mode, the reconstruction of fluorescent lifetime is transformed into a relatively simple optimization problem. This algorithm helps overcome the ill-posedness with FMT. The effectiveness of the proposed method is numerically demonstrated using a heterogeneous mouse chest phantom, showing good accuracy, stability, noise characteristics and computational efficiency.

© 2005 Optical Society of America

OCIS Codes
(110.6960) Imaging systems : Tomography
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Research Papers

History
Original Manuscript: September 6, 2005
Revised Manuscript: September 1, 2005
Published: November 28, 2005

Citation
Alexander Cong and Ge Wang, "A finite-element-based reconstruction method for 3D fluorescence tomography," Opt. Express 13, 9847-9857 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-24-9847


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