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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 26 — Dec. 26, 2005
  • pp: 10440–10447

Investigation of polarization effects for high-numerical-aperture first-order Laguerre-Gaussian beams by 2D scanning with a single fluorescent microbead

Nándor Bokor, Yoshinori Iketaki, Takeshi Watanabe, and Masaaki Fujii  »View Author Affiliations

Optics Express, Vol. 13, Issue 26, pp. 10440-10447 (2005)

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The focal intensity distribution of strongly focused (NA=0.9) first-order Laguerre-Gaussian doughnut beams is investigated experimentally for three different polarizations: linear, and left-handed circular and right-handed circular. The investigations are done by 2-dimensional scanning the focal plane with of a 100nm diameter fluorescent microbead, and measuring the fluorescence signal. The results are shown to be in excellent agreement with theoretical predictions, and demonstrate the superiority of one of the circular polarizations to achieve a sharp dark central spot.

© 2005 Optical Society of America

OCIS Codes
(050.1960) Diffraction and gratings : Diffraction theory
(180.2520) Microscopy : Fluorescence microscopy
(220.2560) Optical design and fabrication : Propagating methods

ToC Category:
Research Papers

Virtual Issues
Vol. 1, Iss. 1 Virtual Journal for Biomedical Optics

Nandor Bokor, Yoshinor Iketaki, Takeshi Watanabe, and Masaaki Fujii, "Investigation of polarization effects for high-numerical-aperture first-order Laguerre-Gaussian beams by 2D scanning with a single fluorescent microbead," Opt. Express 13, 10440-10447 (2005)

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