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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 26 — Dec. 26, 2005
  • pp: 10665–10672

Membrane ripples of a living cell measured by non-interferometric widefield optical profilometry

Chun-Chieh Wang, Jiunn-Yuan Lin, and Chau-Hwang Lee  »View Author Affiliations


Optics Express, Vol. 13, Issue 26, pp. 10665-10672 (2005)
http://dx.doi.org/10.1364/OPEX.13.010665


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Abstract

We measured the membrane topography and dynamics on a living fibroblast by using the non-interferometric widefield optical profilometry (NIWOP) technique. With a water-immersion objective of a 0.75 numerical aperture, our NIWOP system provides depth resolution about 20 nm. The imaging speed could be as high as 5 frames/min. We directly observed and profiled the inward propagation of membrane ripples near the cell edge. To verify if the membrane activity was driven by the underlying cytoskeleton, we changed the structure of the cell cortex while observing the membrane topography. After dissolving the actin cortex by cytochalasin D, we found that the propagation of the membrane ripples disappeared and the edge of the cell shank. The non-contact NIWOP technique does not affect the motility and viability of cells and therefore is suitable for the studies on cell physiology related to membrane motions.

© 2005 Optical Society of America

OCIS Codes
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Research Papers

Virtual Issues
Vol. 1, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Chun-Chieh Wang, Jiunn-Yuan Lin, and Chau-Hwang Lee, "Membrane ripples of a living cell measured by non-interferometric widefield optical profilometry," Opt. Express 13, 10665-10672 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-26-10665


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References

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