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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 3 — Feb. 7, 2005
  • pp: 689–694

Spiral phase contrast imaging in microscopy

Severin Fürhapter, Alexander Jesacher, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations

Optics Express, Vol. 13, Issue 3, pp. 689-694 (2005)

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We demonstrate an optical method for edge contrast enhancement in light microscopy. The method is based on holographic Fourier plane filtering of the microscopic image with a spiral phase element (also called vortex phase or helical phase filter) displayed as an off-axis hologram at a computer controlled high resolution spatial light modulator (SLM) in the optical imaging pathway. The phase hologram imprints a helical phase term of the form exp() on the diffracted light field in its Fourier plane. In the image plane, this results in a strong and isotropic edge contrast enhancement for both amplitude and phase objects.

© 2005 Optical Society of America

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(090.1970) Holography : Diffractive optics
(100.5090) Image processing : Phase-only filters

ToC Category:
Research Papers

Original Manuscript: December 1, 2004
Revised Manuscript: January 10, 2005
Published: February 7, 2005

Severin Fürhapter, Alexander Jesacher, Stefan Bernet, and Monika Ritsch-Marte, "Spiral phase contrast imaging in microscopy," Opt. Express 13, 689-694 (2005)

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