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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 3 — Feb. 7, 2005
  • pp: 702–715

Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo-a preliminary study

Michal Heger, Johan F. Beek, Karin Stenback, Dirk J. Faber, Martin J.C. van Gemert, and Can Ince  »View Author Affiliations

Optics Express, Vol. 13, Issue 3, pp. 702-715 (2005)

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Due to the limited number of suitable intravital microscopy techniques, relatively little is known about the opto-thermal (endo)vascular responses to selective photothermolysis, used as a default treatment modality for superficial vascular anomalies such as port wine stains, telangiectasias, and hemangiomas. In this preliminary study we present a novel microscopy technique for studying (endo)vascular laser-tissue interactions in vivo, in which conventional orthogonal polarized spectral (OPS) imaging is combined with darkfield (DF) illumination. DFOPS imaging of rat mesenteric vasculature irradiated at increasing powers revealed the following (tissular) responses: formation of translucent aggregates, retrograde flow, gradual and immediate hemostasis, reinstatement of flow, vessel disappearance, and perivascular collagen damage. DFOPS imaging therefore constitutes a useful tool for examining (endo)vascular events following selective photothermolysis.

© 2005 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0110) Medical optics and biotechnology : Imaging systems
(350.5340) Other areas of optics : Photothermal effects

ToC Category:
Research Papers

Original Manuscript: December 9, 2004
Revised Manuscript: January 14, 2005
Published: February 7, 2005

Michal Heger, Johan Beek, Karin Stenback, Dirk Faber, Martin van Gemert, and Can Ince, "Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo �?? a preliminary study," Opt. Express 13, 702-715 (2005)

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