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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 4 — Feb. 21, 2005
  • pp: 1113–1123

Force detection in optical tweezers using backscattered light

J. H. G. Huisstede, K. O. van der Werf, M. L. Bennink, and V. Subramaniam  »View Author Affiliations

Optics Express, Vol. 13, Issue 4, pp. 1113-1123 (2005)

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In force-measuring optical tweezers applications the position of a trapped bead in the direction perpendicular to the laser beam is usually accurately determined by measuring the deflection of the light transmitted through the bead. In this paper we demonstrate that this position and thus the force exerted on the bead can be determined using the backscattered light. Measuring the deflection for a 2.50µm polystyrene bead with both a position sensitive detector (PSD) and a quadrant detector (QD) we found that the linear detection range for the PSD is approximately twice that for the QD. In a transmission-based setup no difference was found between both detector types. Using a PSD in both setups the linear detection range for 2.50µm beads was found to be approximately 0.50µm in both cases. Finally, for the reflection-based setup, parameters such as deflection sensitivity and linear detection range were considered as a function of bead diameter (in the range of 0.5–2.5µm). 140pN was the largest force obtained using 2.50µm beads.

© 2005 Optical Society of America

OCIS Codes
(120.4640) Instrumentation, measurement, and metrology : Optical instruments
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(290.1350) Scattering : Backscattering

ToC Category:
Research Papers

Original Manuscript: December 14, 2004
Revised Manuscript: December 14, 2004
Published: February 21, 2005

J. Huisstede, K. van der Werf, M. Bennink, and V. Subramaniam, "Force detection in optical tweezers using backscattered light," Opt. Express 13, 1113-1123 (2005)

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