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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 5 — Mar. 7, 2005
  • pp: 1468–1476

Scanningless depth-resolved microscopy

Dan Oron, Eran Tal, and Yaron Silberberg  »View Author Affiliations

Optics Express, Vol. 13, Issue 5, pp. 1468-1476 (2005)

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The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. This method is applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers with nearly 105 effective pixels using a standard Ti:Sapphire laser oscillator.

© 2005 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(180.6900) Microscopy : Three-dimensional microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Research Papers

Original Manuscript: January 5, 2005
Revised Manuscript: February 2, 2005
Published: March 7, 2005

Dan Oron, Eran Tal, and Yaron Silberberg, "Scanningless depth-resolved microscopy," Opt. Express 13, 1468-1476 (2005)

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