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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 5 — Mar. 7, 2005
  • pp: 1555–1560

Optically-controllable, micron-sized motor based on live cells

M. Gudipati, J. S. D’Souza, J. A. Dharmadhikari, A. K. Dharmadhikari, B. J. Rao, and D. Mathur  »View Author Affiliations


Optics Express, Vol. 13, Issue 5, pp. 1555-1560 (2005)
http://dx.doi.org/10.1364/OPEX.13.001555


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Abstract

We demonstrate rotation of live Chlamydomonas reinhardtii cells in an optical trap; the speed and direction of rotation are amenable to control by varying the optical trapping force. Cells rotate with a frequency of 60–100 rpm; functional flagella are shown to play a decisive role in rotation. The rotating cells generate torque (typically ~7500–12000 pN nm) that is much larger than that generated chemically by a dynein head in vitro (40 pN nm). The total force associated with a rotating live cell (~10 pN) suggests that activity of only a small fraction (~5%) of dynein molecules per beat cycle is sufficient to generate flagellar motion.

© 2005 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:
Research Papers

History
Original Manuscript: December 3, 2004
Revised Manuscript: February 21, 2005
Published: March 7, 2005

Citation
M. Gudipati, J. D'Souza, J. Dharmadhikari, A. Dharmadhikari, B. Rao, and D. Mathur, "Optically-controllable, micron-sized motor based on live cells," Opt. Express 13, 1555-1560 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-5-1555


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References

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