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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 7 — Apr. 4, 2005
  • pp: 2337–2344

Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents

R. Cicchi, F. S. Pavone, D. Massi, and D. D. Sampson  »View Author Affiliations

Optics Express, Vol. 13, Issue 7, pp. 2337-2344 (2005)

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We investigate the application of hyperosmotic optical clearing agents to improve the image contrast and penetration depth in two-photon microscopy of human dermis ex vivo. We show that the agents glycerol, propylene glycol, and glucose all convey significant improvements and we provide results on their dynamic behaviour and the reversibility of the effect. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging.

© 2005 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.5810) Medical optics and biotechnology : Scanning microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Research Papers

Original Manuscript: February 8, 2005
Revised Manuscript: March 15, 2005
Published: April 4, 2005

R. Cicchi, D. Sampson, D. Massi, and F. Pavone, "Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents," Opt. Express 13, 2337-2344 (2005)

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