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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 7 — Apr. 4, 2005
  • pp: 2564–2577

Time-dependent whole-body fluorescence tomography of probe bio-distributions in mice

Sachin V. Patwardhan, Sharon R. Bloch, Samuel Achilefu, and Joseph P. Culver  »View Author Affiliations


Optics Express, Vol. 13, Issue 7, pp. 2564-2577 (2005)
http://dx.doi.org/10.1364/OPEX.13.002564


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Abstract

We present a fast scanning fluorescence optical tomography system for imaging the kinetics of probe distributions through out the whole body of small animals. Configured in a plane parallel geometry, the system scans a source laser using a galvanometer mirror pair (τswitch~1ms) over flexible source patterns, and detects excitation and emission light using a high frame rate low noise, 5 MHz electron multiplied charge-coupled device (EMCCD) camera. Phantom studies were used to evaluate resolution, linearity, and sensitivity. Time dependent (δt=2.2 min.) in vivo imaging of mice was performed following injections of a fluorescing probe (indocyanine green). The capability to detect differences in probe delivery route was demonstrated by comparing an intravenous injection, versus an injection into a fat pocket (retro orbital injection). Feasibility of imaging the distribution of tumor-targeted molecular probes was demonstrated by imaging a breast tumor-specific near infrared polypeptide in MDA MB 361 tumor bearing nude mice. A tomography scan, at 24 hour post injection, revealed preferential uptake in the tumor relative to surrounding tissue.

© 2005 Optical Society of America

OCIS Codes
(110.6960) Imaging systems : Tomography
(260.2510) Physical optics : Fluorescence

ToC Category:
Research Papers

History
Original Manuscript: January 20, 2005
Revised Manuscript: March 19, 2005
Published: April 4, 2005

Citation
Sachin Patwardhan, Sharon Bloch, Samuel Achilefu, and Joseph Culver, "Time-dependent whole-body fluorescence tomography of probe bio-distributions in mice," Opt. Express 13, 2564-2577 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-7-2564


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