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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 8 — Apr. 18, 2005
  • pp: 2852–2857

Real-time interactive 3D manipulation of particles viewed in two orthogonal observation planes

Ivan R. Perch-Nielsen, Peter John Rodrigo, and Jesper Glückstad  »View Author Affiliations

Optics Express, Vol. 13, Issue 8, pp. 2852-2857 (2005)

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The generalized phase contrast (GPC) method has been applied to transform a single TEM00 beam into a manifold of counterpropagating-beam traps capable of real-time interactive manipulation of multiple microparticles in three dimensions (3D). This paper reports on the use of low numerical aperture (NA), non-immersion, objective lenses in an implementation of the GPC-based 3D trapping system. Contrary to high-NA based optical tweezers, the GPC trapping system demonstrated here operates with long working distance (>10 mm), and offers a wider manipulation region and a larger field of view for imaging through each of the two opposing objective lenses. As a consequence of the large working distance, simultaneous monitoring of the trapped particles in a second orthogonal observation plane is demonstrated.

© 2005 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(180.0180) Microscopy : Microscopy
(230.6120) Optical devices : Spatial light modulators

ToC Category:
Research Papers

Original Manuscript: February 28, 2005
Revised Manuscript: March 30, 2005
Published: April 18, 2005

Ivan Perch-Nielsen, Peter Rodrigo, and Jesper Glückstad, "Real-time interactive 3D manipulation of particles viewed in two orthogonal observation planes," Opt. Express 13, 2852-2857 (2005)

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