Reconstruction of complementary images in second harmonic generation microscopy

doi:10.1364/OE.14.004727

Reconstruction of complementary images in second harmonic generation microscopy

Liang Gao, Lei Jin, Ping Xue, Jun Xu, Yi Wang, Hui Ma, and Dieyan Chen »View Author Affiliations

Optics Express, Vol. 14, Issue 11, pp. 4727-4735 (2006)
http://dx.doi.org/10.1364/OE.14.004727

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Abstract

Second harmonic generation microscopy(SHGM) has become widely used to image biological samples. Due to the complexity of biological samples, more and more effort has been put on polarization imaging in SHGM technology to uncover their structures. In this work, we put forward a novel stitching method based on careful mathematical calculation, and accomplish it by rotating laser polarization. We first show its validity in imaging a perfectly synthesized bio-origin polymer poly (3-hyroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Then, we test its power by getting a true image of fibrillar collagen structure of rat-tail tendon.

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 27, 2006
Revised Manuscript: May 5, 2006
Manuscript Accepted: May 9, 2006
Published: May 29, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Liang Gao, Lei Jin, Ping Xue, Jun Xu, Yi Wang, Hui Ma, and Dieyan Chen, "Reconstruction of complementary images in second harmonic generation microscopy," Opt. Express 14, 4727-4735 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-11-4727

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References

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