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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 12 — Jun. 12, 2006
  • pp: 5191–5200

Graded-field microscopy with white light

Ran Yi, Kengyeh K. Chu, and Jerome Mertz  »View Author Affiliations

Optics Express, Vol. 14, Issue 12, pp. 5191-5200 (2006)

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We present a general imaging technique called graded-field microscopy for obtaining phase-gradient contrast in biological tissue slices. The technique is based on introducing partial beam blocks in the illumination and detection apertures of a standard white-light widefield transillumination microscope. Depending on the relative aperture sizes, one block produces phase-gradient contrast while the other reduces brightfield background, allowing a full operating range between brightfield and darkfield contrast. We demonstrate graded-field imaging of neurons in a rat brain slice.

© 2006 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.1220) Imaging systems : Apertures
(110.4980) Imaging systems : Partial coherence in imaging

ToC Category:
Imaging Systems

Original Manuscript: May 4, 2006
Revised Manuscript: May 30, 2006
Manuscript Accepted: May 30, 2006
Published: June 12, 2006

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Ran Yi, Kengyeh K. Chu, and Jerome Mertz, "Graded-field microscopy with white light," Opt. Express 14, 5191-5200 (2006)

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