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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 13 — Jun. 26, 2006
  • pp: 6178–6187

In vivo optical biopsy of hamster oral cavity with epi-third-harmonic-generation microscopy

Shih-Peng Tai, Wen-Jeng Lee, Dar-Bin Shieh, Ping-Ching Wu, Hsin-Yi Huang, Che-Hang Yu, and Chi-Kuang Sun  »View Author Affiliations

Optics Express, Vol. 14, Issue 13, pp. 6178-6187 (2006)

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The first in vivo optical virtual biopsy based on epi-third-harmonic-generation (THG) microscopy is successfully demonstrated using Syrian hamster oral mucosa as a model system. Without complex physical biopsy procedures, epi-THG microscopy can provide high spatial resolution dynamic images of oral mucosa and sub-mucosa in all three dimensions. The demonstrated intra-vital epi-THG microscopy provide high resolution observation of blood flow in the capillary and could be a promising tool to image angiogenesis, which is an important feature for many human diseases including malignancies. The system setup of epi-THG microscopy can be easily integrated with other nonlinear optical microscopy such as second-harmonic generation and multi-photon fluorescence microscopy by using the same laser system to provide better integrated molecular and structural information for future clinical diagnosis. By adding 6% acetic acid solution on the mucosa, THG contrast on the borders of nuclei was found to be greatly enhanced due to the alterations of their linear and nonlinear THG susceptibilities. With a virtual-transition-based technology without using fluorescence, the optical epi-THG biopsy we demonstrated shows promise for future noninvasive in vivo diseases examinations.

© 2006 Optical Society of America

OCIS Codes
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

ToC Category:

Original Manuscript: May 15, 2006
Revised Manuscript: June 6, 2006
Manuscript Accepted: June 8, 2006
Published: June 26, 2006

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Shih-Peng Tai, Wen-Jeng Lee, Dar-Bin Shieh, Ping-Ching Wu, Hsin-Yi Huang, Che-Hang Yu, and Chi-Kuang Sun, "In vivo optical biopsy of hamster oral cavity with epi-third-harmonic-generation microscopy," Opt. Express 14, 6178-6187 (2006)

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