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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 14 — Jul. 10, 2006
  • pp: 6535–6542

Epi-illumination through the microscope objective applied to darkfield imaging and microspectroscopy of nanoparticle interaction with cells in culture

Adam Curry, William L. Hwang, and Adam Wax  »View Author Affiliations

Optics Express, Vol. 14, Issue 14, pp. 6535-6542 (2006)

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Existing darkfield illumination schemes are incompatible with many types of samples and/or procedures. We present a darkfield epi-illumination scheme which addresses these incompatibilities by providing illumination through the imaging objective. We validate the system performance using silver nanospheres in varying refractive index environments, characterize the intensity distribution of the darkfield illumination, and demonstrate system capabilities through a preliminary study of functionalized gold nanosphere interactions with cancer cells in culture. We observe a broadened scattering spectrum from unconjugated nanoparticles, as compared with anti-EGFR conjugated nanoparticles, upon incubation with cancer cells, and discuss the implications of this observation.

© 2006 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(290.0290) Scattering : Scattering
(300.0300) Spectroscopy : Spectroscopy
(350.4990) Other areas of optics : Particles

ToC Category:

Original Manuscript: April 21, 2006
Revised Manuscript: June 19, 2006
Manuscript Accepted: June 19, 2006
Published: July 10, 2006

Virtual Issues
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics

Adam Curry, William L. Hwang, and Adam Wax, "Epi-illumination through the microscope objective applied to darkfield imaging and microspectroscopy of nanoparticle interaction with cells in culture," Opt. Express 14, 6535-6542 (2006)

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