OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 15 — Jul. 24, 2006
  • pp: 6713–6723

In-vivo NIR autofluorescence imaging of rat mammary tumors

Laure S. Fournier, Vincenzo Lucidi, Kirill Berejnoi, Theodore Miller, Stavros G. Demos, and Robert C. Brasch  »View Author Affiliations


Optics Express, Vol. 14, Issue 15, pp. 6713-6723 (2006)
http://dx.doi.org/10.1364/OE.14.006713


View Full Text Article

Enhanced HTML    Acrobat PDF (493 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We investigate in vivo detection of mammary tumors in a rat model using autofluorescence imaging in the red and far-red spectral regions. The objective was to explore this method for non-invasive detection of malignant tumors and correlation between autofluorescence properties of tumors and their pathologic status. Eighteen tumor-bearing rats, bearing eight benign and seventeen malignant tumors were imaged. Autofluorescence images were acquired using spectral windows centered at 700-nm, 750-nm and 800-nm under laser excitation at 632.8-nm and 670-nm. Intensity in the autofluorescence images of malignant tumors under 670-nm excitation was higher than that of the adjacent normal tissue. whereas intensity of benign tumors was lower compared to normal tissue.

© 2006 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(110.3080) Imaging systems : Infrared imaging
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 9, 2006
Revised Manuscript: June 21, 2006
Manuscript Accepted: June 22, 2006
Published: July 24, 2006

Virtual Issues
Vol. 1, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Laure S. Fournier, Vincenzo Lucidi, Kirill Berejnoi, Theodore Miller, Stavros G. Demos, and Robert C. Brasch, "In-vivo NIR autofluorescence imaging of rat mammary tumors," Opt. Express 14, 6713-6723 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-15-6713


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. National Cancer Institute, "Cancernet resource" (National Cancer Institute, 2003), http://cis.nci.nih.gov/fact/5_6.htm.
  2. National Cancer Institute, "Surveillance, Epidemiology, and End Results: Estimated new cancer cases and deaths for 2004" (National Cancer Institute, 2004), http://seer.cancer.gov/cgi-bin/csr/1975_2001/search.pl#results
  3. T. M. Kolb, J. Lichy and J. H. Newhouse, "Comparison of the performance of screening mammography, physical examination, and breast US and evaluation of factors that influence them: an analysis of 27,825 patient evaluations," Radiology 225, 165-75 (2002). [CrossRef] [PubMed]
  4. M. Cutler, "Transillumination as an aid in the diagnosis of breast lesions.," Surg. Gynecol. Obstet. 48,721-729 (1929).
  5. X. Gu, Q. Zhang, M. Bartlett, L. Schutz, L. L. Fajardo and H. Jiang, "Differentiation of cysts from solid tumors in the breast with diffuse optical tomography," Acad. Radiol. 11,53-60 (2004). [CrossRef] [PubMed]
  6. D. B. Jakubowski, A. E. Cerussi, F. Bevilacqua, N. Shah, D. Hsiang, J. Butler and B. J. Tromberg, "Monitoring neoadjuvant chemotherapy in breast cancer using quantitative diffuse optical spectroscopy: a case study," J. Biomed. Opt. 9,230-8 (2004). [CrossRef] [PubMed]
  7. V. Ntziachristos, A. G. Yodh, M. Schnall and B. Chance, "Concurrent MRI and diffuse optical tomography of breast after indocyanine green enhancement," Proc. Natl. Acad. Sci. U. S. A. 97,2767-72. (2000). [CrossRef] [PubMed]
  8. G. A. Wagnieres, W. M. Star and B. C. Wilson, "In vivo fluorescence spectroscopy and imaging for oncological applications," Photochem. Photobiol. 68,603-32. (1998). [PubMed]
  9. C. J. Frank, D. C. Redd, T. S. Gansler and R. L. McCreery, "Characterization of human breast biopsy specimens with near-IR Raman spectroscopy," Anal. Chem. 66,319-26 (1994). [CrossRef] [PubMed]
  10. A. S. Haka, K. E. Shafer-Peltier, M. Fitzmaurice, J. Crowe, R. R. Dasari and M. S. Feld, "Identifying microcalcifications in benign and malignant breast lesions by probing differences in their chemical composition using Raman spectroscopy," Cancer Res. 62,5375-80 (2002). [PubMed]
  11. R. Manoharan, K. Shafer, L. Perelman, J. Wu, K. Chen, G. Deinum, M. Fitzmaurice, J. Myles, J. Crowe, R. R. Dasari and M. S. Feld, "Raman spectroscopy and fluorescence photon migration for breast cancer diagnosis and imaging," Photochem. Photobiol. 67,15-22 (1998). [CrossRef] [PubMed]
  12. S. G. Demos, H. Savage, A. S. Heerdt, S. Schantz and R. R. Alfano, "Time Resolved Degree of Polarization for Human Breast Tissue," Optics Commun. 124,439 (1996). [CrossRef]
  13. D. W. Chicken, A. C. Lee, G. M. Briggs, M. R. S. Keshtgar, K. S. Johnson, D. D. O. Pickard, I. J. Bigio and S. G. Bown, "Optical biopsy: A novel intraoperative diagnostic tool to determine sentinel lymph node status instantly in breast cancer," Breast Cancer Res. Treat. 82,S172-S174 (2003).
  14. Demos, S. G. , R. Gandour-Edwards, R. Ramsamooj and R. DeVere White, "Spectroscopic detection of bladder cancer using near-infrared imaging techniques," J. Biomed. Opt. 9, 767-71 (2004). [CrossRef] [PubMed]
  15. S. G. Demos, R. Gandour-Edwards, R. Ramsamooj and R. White, "Near-infrared autofluorescence imaging for detection of cancer," J. Biomed. Opt. 9,587-92 (2004). [CrossRef] [PubMed]
  16. G. Zhang, S. G. Demos and R. R. Alfano, "Far-red and NIR spectral wing emission from tissues under 532-nm and 632-nm photo-excitation," Lasers Life Sci. 9,1-16 (1999).
  17. M. Inaguma and K. Hashimoto, "Porphyrin-like fluorescence in oral cancer: In vivo fluorescence spectral characterization of lesions by use of a near-ultraviolet excited autofluorescence diagnosis system and separation of fluorescent extracts by capillary electrophoresis," Cancer 86,2201-11. (1999). [CrossRef] [PubMed]
  18. G. Stoica and A. Koestner, "Diverse spectrum of tumors in male Sprague-Dawley rats following single high doses of N-ethyl-N-nitrosourea (ENU)," Am. J. Pathol. 116,319-26 (1984). [PubMed]
  19. R. Scarff and H. Torloni, "Histological typing of breast tumors," in World Health Organization, (Geneva, 1968), pp. 13-20.
  20. M. M. el-Sharabasy, A. M. el-Waseef, M. M. Hafez and S. A. Salim, "Porphyrin metabolism in some malignant diseases," Br. J. Cancer 65,409-12. (1992). [CrossRef] [PubMed]
  21. L. Rasetti, G. F. Rubino, L. Tettinatti and G. W. Drago, "Porphyrin porphobilinogen and amino ketone levels in tumor tissue," Panminerva Med. 7,105-110 (1965).
  22. B. Zawirska, "Comparative porphyrin content in tumors with contiguous non-neoplastic tissues," Neoplasma 26,223-9. (1979). [PubMed]
  23. T. Theodossiou and A. J. MacRobert, "Comparison of the photodynamic effect of exogenous photoprotoporphyrin and protoporphyrin IX on PAM 212 murine keratocytes.," Photochem. Photobiol. 76,530-537 (2002). [CrossRef] [PubMed]
  24. D. M. Harris and J. Werkhaven, "Endogenous porphyrin fluorescence in tumors," Lasers Surg. Med. 7,467-72. (1987). [CrossRef] [PubMed]
  25. H. Daldrup, D. M. Shames, M. Wendland, Y. Okuhata, T. M. Link, W. Rosenau, Y. Lu and R. C. Brasch, "Correlation of dynamic contrast-enhanced MR imaging with histologic tumor grade: comparison of macromolecular and small-molecular contrast media," AJR Am. J. Roentgenol. 171,941-9. (1998). [PubMed]
  26. M. Y. Su, Z. Wang, P. M. Carpenter, X. Lao, A. Muhler and O. Nalcioglu, "Characterization of N-ethyl-N-nitrosourea-induced malignant and benign breast tumors in rats by using three MR contrast agents," J. Magn. Reson. 9,177-86 (1999). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 
Fig. 4.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited