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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 16 — Aug. 7, 2006
  • pp: 7005–7013

Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

Florian Charrière, Nicolas Pavillon, Tristan Colomb, Christian Depeursinge, Thierry J. Heger, Edward A.D. Mitchell, Pierre Marquet, and Benjamin Rappaz  »View Author Affiliations

Optics Express, Vol. 14, Issue 16, pp. 7005-7013 (2006)

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This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of π, allowing to built 3-dimensional quantitative refractive index distributions by an inverse Radon transform. A 20× magnification allows a resolution better than 3 μm in all three dimensions, with accuracy better than 0.01 for the refractive index measurements. This technique is for the first time to our knowledge applied to living specimen (testate amoeba, Protista). Morphometric measurements are extracted from the tomographic reconstructions, showing that the commonly used method for testate amoeba biovolume evaluation leads to systematic under evaluations by about 50%.

© 2006 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(110.6880) Imaging systems : Three-dimensional image acquisition
(110.6960) Imaging systems : Tomography
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy

ToC Category:

Original Manuscript: June 9, 2006
Revised Manuscript: July 20, 2006
Manuscript Accepted: July 24, 2006
Published: August 7, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Florian Charrière, Nicolas Pavillon, Tristan Colomb, Christian Depeursinge, Thierry J. Heger, Edward A. D. Mitchell, Pierre Marquet, and Benjamin Rappaz, "Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba," Opt. Express 14, 7005-7013 (2006)

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