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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 16 — Aug. 7, 2006
  • pp: 7125–7133

Femtosecond optical transfection of cells:viability and efficiency

D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia  »View Author Affiliations


Optics Express, Vol. 14, Issue 16, pp. 7125-7133 (2006)
http://dx.doi.org/10.1364/OE.14.007125


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Abstract

Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 μJ/cm2, have found an average transfection efficiency of 50 ± 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.

© 2006 Optical Society of America

OCIS Codes
(140.0140) Lasers and laser optics : Lasers and laser optics
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(320.7090) Ultrafast optics : Ultrafast lasers

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 7, 2006
Revised Manuscript: June 8, 2006
Manuscript Accepted: June 13, 2006
Published: August 7, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Citation
D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia, "Femtosecond optical transfection of cells:viability and efficiency," Opt. Express 14, 7125-7133 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-16-7125


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