OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 16 — Aug. 7, 2006
  • pp: 7134–7143

Optics InfoBase > Optics Express > Volume 14 > Issue 16 > Page 7134

Fluorescence coherence tomography

A. Bilenca, A. Ozcan, B. Bouma, and G. Tearney  »View Author Affiliations


Optics Express, Vol. 14, Issue 16, pp. 7134-7143 (2006)
http://dx.doi.org/10.1364/OE.14.007134


View Full Text Article

Enhanced HTML    Acrobat PDF (2634 KB)


Advanced Search

Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

In this paper, we introduce a new form of cross-sectional, coherence-gated fluorescence imaging, which we term ‘spectral-domain fluorescence coherence tomography’ (SD-FCT). SD-FCT is accomplished by spectrally detecting self-interference of the spontaneous emission of fluorophores located along the axial (depth) dimension of the sample. We have built a first generation SD-FCT system that utilizes two opposing low numerical-aperture objective lenses in an interferometer and an imaging spectrometer for detecting self-interference of fluorescence emitted from a sample. Here, in proof-of-principle experiments we demonstrate cross-sectional profiling of layered fluorescence phantoms. Narrow (a few micrometers FWHM) axial point-spread functions, large ranging depths (a few hundreds of micrometers) and wide fields of view (>1 mm) were measured. Initial results suggest that SD-FCT may be a viable tool for the investigation of semi-transparent and selectively labeled fluorescent samples.

© 2006 Optical Society of America

OCIS Codes
(030.1670) Coherence and statistical optics : Coherent optical effects
(170.6960) Medical optics and biotechnology : Tomography
(260.2510) Physical optics : Fluorescence

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 13, 2006
Revised Manuscript: July 19, 2006
Manuscript Accepted: July 25, 2006
Published: August 7, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Citation
A. Bilenca, A. Ozcan, B. Bouma, and G. Tearney, "Fluorescence coherence tomography," Opt. Express 14, 7134-7143 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-16-7134


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991). [CrossRef] [PubMed]
  2. A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, "Optical coherence tomography - principles and applications," Rep. Prog. Phys. 66, 239-303 (2003). [CrossRef]
  3. J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, "Optical coherence microscopy in scattering media," Opt. Lett. 19, 590-592 (1994). [CrossRef] [PubMed]
  4. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, "Optical sectioning deep inside live embryos by selective plane illumination microscopy," Science 305, 1007-1009 (2004). [CrossRef] [PubMed]
  5. K. H. Drexhage, "Interaction of light with monomolecular dye layers," Prog. Opt. 12, 163-232 (1974). [CrossRef]
  6. K. E. Drabe, G. Cnossen, and D. A. Wiersma, "Localization of spontaneous emission in front of a mirror," Opt. Commun. 73, 91-95 (1989). [CrossRef]
  7. A. Lambacher and P. Fromherz, "Fluorescence interference-contrast microscopy on oxidized silicon using a monomolecular dye layer," Appl. Phys. A 63, 207-216 (1996). [CrossRef]
  8. A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, M. S. Unlu, "Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference," IEEE J. Sel. Top. Quantum Electron. 9, 294-300 (2003). [CrossRef]
  9. S. Hell and E. H. K. Stelzer, "Properties of a 4Pi-confocal fluorescence microscope," J. Opt. Soc. Am. A 9, 2159-2166 (1992). [CrossRef]
  10. M. G. L. Gustafsson, D. A. Agard and J. W. Sedat, "Sevenfold improvement of axial resolution in 3D widefiled microscopy using two objective lenses," in Three-dimensional Microscopy: Image Acquisition and Processing II, Tony Wilson and Carol J. Cogswell, eds., 2412, 147-156 (1995).
  11. B. Karamata, P. Lambelet, M. Laubscher, R. P. Salathé, and T. Lasser, "Spatially incoherent illumination as a mechanism for cross-talk suppression in wide-field optical coherence tomography," Opt. Lett. 29, 736-738 (2004). [CrossRef] [PubMed]
  12. M. Wojtkowski, A. Kowalczyk, R. Leitgeb, and A. F. Fercher, "Full range complex spectral optical coherence tomography technique in eye imaging," Opt. Lett. 27, 1415-1417 (2002). [CrossRef]
  13. B. Grajciar, M. Pircher, A. Fercher, and R. Leitgeb, "Parallel Fourier domain optical coherence tomography for in vivo measurement of the human eye," Opt. Express 13, 1131-1137 (2005). [CrossRef] [PubMed]
  14. C. Dorrer, N. Belabas, J-P Likforman, and M. Joffre, "Spectral resolution and sampling issues in Fourier transform spectral interferometry," J. Opt. Soc. Am. B 17, 1795-1802 (2000). [CrossRef]
  15. N. Nassif, B. Cense, B. Park, M. Pierce, S. Yun, B. Bouma, G. Tearney, T. Chen, and J. de Boer, "In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve," Opt. Express 12, 367-376 (2004). [CrossRef] [PubMed]
  16. S. Yun, G. Tearney, B. Bouma, B. Park, and J. de Boer, "High-speed spectral-domain optical coherence tomography at 1.3 μm wavelength," Opt. Express 11, 3598-3604 (2003). [CrossRef] [PubMed]
  17. A. Bilenca, A. Desjardins, B. Bouma, and G. Tearney, "Multicanonical Monte-Carlo simulations of light propagation in biological media," Opt. Express 13, 9822-9833 (2005). [CrossRef] [PubMed]

Cited By

 Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited