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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 16 — Aug. 7, 2006
  • pp: 7420–7435

A primary method for determination of optical parameters of turbid samples and application to intralipid between 550 and 1630nm

Cheng Chen, Jun Q. Lu, Huafeng Ding, Kenneth M. Jacobs, Yong Du, and Xin-Hua Hu  »View Author Affiliations

Optics Express, Vol. 14, Issue 16, pp. 7420-7435 (2006)

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The lack of a primary method for determination of optical parameters remains a significant barrier in optical study of turbid media. We present a complete system of experimental setups and Monte Carlo modeling tools for fast and accurate solution of the inverse problem from the measured signals of homogeneous turbid samples. The calibration of the instrument and validation of the Monte Carlo modeling have been carried out to ensure the accuracy of the inverse solution. We applied this method to determine the optical parameters of turbid media of 10% intralipid between 550 and 940nm and 20% intralipid between 550 and 1630nm.

© 2006 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(290.7050) Scattering : Turbid media

ToC Category:

Original Manuscript: May 1, 2006
Revised Manuscript: June 28, 2006
Manuscript Accepted: July 3, 2006
Published: August 7, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Cheng Chen, Jun Q. Lu, Huafeng Ding, Kenneth M. Jacobs, Yong Du, and Xin-Hua Hu, "A primary method for determination of optical parameters of turbid samples and application to intralipid between 550 and 1630nm," Opt. Express 14, 7420-7435 (2006)

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  1. H. C. van de Hulst, Multiple light scattering: tables, formulas, and applications, Vol. 1 & 2. (Academic Press, New York, 1980).
  2. M. Born, E. Wolf, and A. B. Bhatia, Principles of optics: electromagnetic theory of propagation, interference and diffraction of light, 7th ed. (Cambridge University Press, Cambridge, England, 1999).
  3. H. Ding, J. Q. Lu, K. M. Jacobs, and X. H. Hu, "Determination of refractive indices of porcine skin tissues and intralipid at eight wavelengths between 325 and 1557nm," J. Opt. Soc. Am. A 22, 1151-1157 (2005). [CrossRef]
  4. L. G. Henyey, and J. L. Greenstein, "Diffuse radiation in the galaxy," Astrophys J 93, 70-83 (1941). [CrossRef]
  5. S. L. Jacques, C. A. Alter, and S. A. Prahl, "Angular dependence of HeNe laser light scattering by human dermis," Lasers Life Sci. 1, 309-333 (1987).
  6. V. G. Peters, D. R. Wyman, M. S. Patterson, and G. L. Frank, "Optical properties of normal and diseased human breast tissues in the visible and near infrared," Phys. Med. Biol. 35, 1317-34 (1990). [CrossRef] [PubMed]
  7. S. A. Prahl, M. J. C. van Gemert,and A. J. Welch, "Determining the optical properties of turbid media by using the adding-doubling method," Appl. Opt. 32, 559-568 (1993). [CrossRef] [PubMed]
  8. I. V. Yaroslavsky, A. N. Yaroslavsky, T. Goldbach, and H.-J. Schwarzmaier, "Inverse hybrid technique for determining the optical properties of turbid media from integrating-sphere measurements," Appl. Opt. 35, 6797-6809 (1996). [CrossRef] [PubMed]
  9. C. K. Hayakawa, B. Y. Hill, J. S. You, F. Bevilacqua, J. Spanier, and V. Venugopalan, "Use of the delta-P1 approximation for recovery of optical absorption, scattering, and asymmetry coefficients in turbid media," Appl Opt 43, 4677-84 (2004). [CrossRef] [PubMed]
  10. A. H. Hielscher, J. R. Mourant,and I. J. Bigio, "Influence of particle size and concentration on the diffuse backscattering of polarized light from tissue phantoms and biological cell suspensions," Appl. Opt. 36, 125-136 (1997). [CrossRef] [PubMed]
  11. Y. Du, X. H. Hu, M. Cariveau, X. Ma, G. W. Kalmus, and J. Q. Lu, "Optical properties of porcine skin dermis between 900 nm and 1500 nm," Phys. Med. Biol. 46, 167-81 (2001). [CrossRef] [PubMed]
  12. X. Ma, J. Q. Lu, R. S. Brock, K. M. Jacobs, P. Yang, and X. H. Hu, "Determination of complex refractive index of Polystyrene Microspheres from 370 to 1610nm," Phys. Med. Biol. 48, 4165-4172 (2003). [CrossRef]
  13. A. Roos, "Interpretation of integrating sphere signal output for nonideal transmitting samples," Appl. Opt. 30, 468-474 (1991). [CrossRef] [PubMed]
  14. J. W. Pickering, S. A. Prahl, N. Vanwieringen, J. F. Beek, H. J. C. M. Sterenborg, and M. J. C. Vangemert, "Double-integrating-sphere system for measuring the optical-properties of tissue," Appl. Opt. 32, 399-410 (1993). [CrossRef] [PubMed]
  15. L. Wang, S. L. Jacques, and L. Zheng, "MCML-Monte Carlo modeling of light transport in multi-layered tissues," Comput Methods Programs Biomed 47, 131-46 (1995). [CrossRef] [PubMed]
  16. S. T. Flock, S. L. Jacques, B. C. Wilson, W. M. Star, and M. J. van Gemert, "Optical properties of Intralipid: a phantom medium for light propagation studies," Lasers Surg. Med. 12, 510-9 (1992). [CrossRef] [PubMed]
  17. S. T. Flock, "The Optical Properties of Tissues and Light Dosimetry," (Hamilton, Ontario, Canada, McMaster University, 1988).
  18. C. J. M. Moes, M. J. C. van Gemert, W. M. Star, J. P. A. Marijnissen, and S. A. Prahl, "Measurements and calculations of the energy fluence rate in a scattering and absorbing phantom at 633 nm," Appl. Opt. 28, 2292-2296 (1989). [CrossRef] [PubMed]
  19. H. G. van Staveren, C. J. M. Moes, J. van Marle, S. A. Prahl, and M. J. C. van Gemert, "Light scattering in Intralipid-10% in the wavelength range of 400-1100 nanometers," Appl. Opt. 30, 4507-4514 (1991). [CrossRef] [PubMed]
  20. S. L. Jacques, "Optical properties of "Intralipid", an aqueous suspension of lipid droplet," (Oregon Medical Laser Center, 1998), http://omlc.ogi.edu/spectra/intralipid/index.html.
  21. C. Chen, J. Q. Lu, and X. H. Hu, "OPDISM - Optical Parameters Determined by Integrating Sphere Measurement," (Biomedical Laser Laboratory, 2006), http://bmlaser.physics.ecu.edu.
  22. M. C. Wang,E. Guth, "On the theory of multiple scattering, particularly of charged particles," Phys. Rev. 84, 1093-1111 (1951). [CrossRef]
  23. A. A. Kokhanovsky, "Small-angle approximations of the radiative transfer theory," J. Phys. D: Appl. Phys. 30, 2837-2840 (1997). [CrossRef]
  24. Z. Song, K. Dong, X. H. Hu, J. Q. Lu, "Monte Carlo simulation of converging laser beams propagating in biological materials," Appl. Opt. 38, 2944-2949 (1999). [CrossRef]
  25. Y. Du, "Optical Properties of Porcine Dermis in the Near Infrared Region between 900nm and 1500nm," Greenville, NC: East Carolina University, 2000, pp. 123.
  26. V. R. Weidner, and J. J. Hsia, "Reflection properties of pressed polytetrafluoroethylene powder," J. Opt. Soc. Am. 71, 856-861 (1981). [CrossRef]
  27. H. Ding, J. Q. Lu, W. A. Wooden, P. J. Kragel, and X. H. Hu, "Refractive indices of human skin tissues at eight wavelengths and estimated dispersion relations between 300 and 1600nm," Phys. Med. Biol. 51 (2006). [CrossRef] [PubMed]
  28. G. Hale, and M. Querry, "Optical constants of water in the 200nm to 200 micrometer wavelength region," Appl. Opt. 12, 555-563 (1973). [CrossRef] [PubMed]
  29. K. Li, J. Q. Lu, R. S. Brock, B. Yang, and X. H. Hu, "Quantitative modeling of skin images using Parallel Monte Carlo Methods," in Advanced Biomedical and Clinical Diagnostic Systems III, T. Vo-Dinh, W. S. Grundfest, D. A. Benaron, and G. E. Cohn, eds., Proc. SPIE 5693,82-87 (2005). [CrossRef]
  30. X. Ma, J. Q. Lu, X. H. Hu, "Effect of surface roughness on determination of bulk tissue optical parameters," Opt. Lett. 28, 2204-6 (2003). [CrossRef] [PubMed]
  31. X. Ma, J. Q. Lu, H. Ding, and X. H. Hu, "Bulk optical parameters of porcine skin dermis tissues at eight wavelengths from 325 to 1557nm," Opt. Lett. 30, 412-414 (2005). [CrossRef] [PubMed]

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