OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 17 — Aug. 21, 2006
  • pp: 7789–7800

In vivo imaging flow cytometer

Ho Lee, Clemens Alt, Costas M. Pitsillides, Mehron Puoris’haag, and Charles P. Lin  »View Author Affiliations


Optics Express, Vol. 14, Issue 17, pp. 7789-7800 (2006)
http://dx.doi.org/10.1364/OE.14.007789


View Full Text Article

Enhanced HTML    Acrobat PDF (1102 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We introduce an in vivo imaging flow cytometer, which provides fluorescence images simultaneously with quantitative information on the cell population of interest in a live animal. As fluorescent cells pass through the slit of light focused across a blood vessel, the excited fluorescence is confocally detected. This cell signal triggers a strobe beam and a high sensitivity CCD camera that captures a snapshot image of the cell as it moves down-stream from the slit. We demonstrate that the majority of signal peaks detected in the in vivo flow cytometer arise form individual cells. The instrument’s capability to image circulating T cells and measure their speed in the blood vessel in real time in vivo is demonstrated. The cell signal irradiance variation, clustering percentage, and potential applications in biology and medicine are discussed.

© 2006 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 4, 2006
Revised Manuscript: June 29, 2006
Manuscript Accepted: July 20, 2006
Published: August 21, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Ho Lee, Clemens Alt, Costas M. Pitsillides, Mehron Puoris'haag, and Charles P. Lin, "In vivo imaging flow cytometer," Opt. Express 14, 7789-7800 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-17-7789


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, "In vivo flow cytometer for real-time detection and quantification of circulating cells," Opt. Lett. 29, 77-79 (2004). [CrossRef] [PubMed]
  2. I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, "In vivo flow cytometry: a new method for enumerating circulating cancer cells," Cancer Res. 64, 5044-5047 (2004). [CrossRef] [PubMed]
  3. X. Wei, D. A. Sipkins, C. M. Pitsillides, J. Novak, I. Georgakoudi, and C. P. Lin, "Real-time detection of circulating apoptotic cells by in vivo flow cytometry," Mol. Imaging. 4, 415-416 (2005). [PubMed]
  4. D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, "In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment," Nature 435, 969-973 (2005). [CrossRef] [PubMed]
  5. V. P. Zharov, E. I. Galanzha, and V. V. Tuchin, "In vivo photothermal flow cytometry: imaging and detection of individual cells in blood and lymph flow," J. Cell. Biochem. 97, 916-932 (2006). [CrossRef] [PubMed]
  6. A. Hafezi-Moghadam, K. L. Thomas, and C. Cornelssen, "A novel mouse-driven ex vivo flow chamber for the study of leukocyte and platelet function," Am. J. Physiol. Cell Physiol. 286, C876-C892 (2004). [CrossRef]
  7. M. Shin, K. Matsuda, O. Ishii, H. Terai, M. Kaazempur-Mofrad, J. Borenstein, M. Detmar, and J. P. Vacanti, "Endothelialized networks with a vascular geometry in microfabricated poly(dimethyl siloxane)," Biomed. Microdevices 6, 269-278 (2004). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited