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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 17 — Aug. 21, 2006
  • pp: 7801–7809

In vivo mouse studies with bioluminescence tomography

Ge Wang, Wenxiang Cong, Kumar Durairaj, Xin Qian, Haiou Shen, Patrick Sinn, Eric Hoffman, Geoffrey McLennan, and Michael Henry  »View Author Affiliations


Optics Express, Vol. 14, Issue 17, pp. 7801-7809 (2006)
http://dx.doi.org/10.1364/OE.14.007801


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Abstract

Bioluminescence tomography (BLT) is a new molecular imaging mode, which is being actively developed to reveal molecular and cellular signatures as labeled by bioluminescent probes in a living small animal. This technology can help diagnose diseases, evaluate therapies, and facilitate drug development with mouse models. In this paper, we describe in vivo mouse experiments with BLT, and propose the reconstruction procedure of bioluminescent sources from optical data measured on the body surface of the mouse using a modality fusion approach. The results show the feasibility of our methodology for localization and quantification of the bioluminescent activities in vivo.

© 2006 Optical Society of America

OCIS Codes
(110.6960) Imaging systems : Tomography
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3660) Medical optics and biotechnology : Light propagation in tissues

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 26, 2006
Revised Manuscript: July 27, 2006
Manuscript Accepted: July 27, 2006
Published: August 21, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Ge Wang, Wenxiang Cong, Kumar Durairaj, Xin Qian, Haiou Shen, Patrick Sinn, Eric Hoffman, Geoffrey McLennan, and Michael Henry, "In vivo mouse studies with bioluminescence tomography," Opt. Express 14, 7801-7809 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-17-7801


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References

  1. V. Ntziachristos, J. Ripoll, L. V. Wang, R. Weissleder, "Looking and listening to light: the evolution of whole-body photonic imaging," Nat. Biotechnol. 23, 313-320 (2005). [CrossRef] [PubMed]
  2. C. Contag and M. H. Bachmann, "Advances in Bioluminescence imaging of gene expression," Annu. Rev. Biomed. Eng. 4, 235-260 (2002). [CrossRef] [PubMed]
  3. W. Rice, M. D. Cable, and M. B. Nelson, "In vivo imaging of light-emitting probes," J. Biomed. Opt. 6, 432-440 (2001). [CrossRef] [PubMed]
  4. G. Wang, E. A. Hoffman, and G. McLennan, Systems and methods for bioluminescent computed tomographic reconstruction. Patent disclosure filled in July 2002; US provisional patent application filled in March 2003; US patent application filed in March 2004.
  5. G. Wang, E. A. Hoffman, G. McLennan, L. V. Wang, M. Suter, and J. Meinel, "Development of the first bioluminescent CT scanner," Radiology 229, 566 (2003).
  6. G. Wang, Y. Li, and M. Jiang, "Uniqueness theorems in bioluminescence tomography," Med. Phys. 31, 2289-2299 (2004). [CrossRef] [PubMed]
  7. W. Cong, D. Kumar, Y. Liu, A. Cong, and G. Wang, "A practical method to determine the light source distribution in bioluminescent imaging," in Developments in X-Ray Tomography IV; U. Bonse, ed., Proc. SPIE 5535, 679-686 (2004). [CrossRef]
  8. W. Cong, G. Wang, D. Kumar, Y. Liu, M. Jiang, L. Wang, E. Hoffman, G. McLennan, P. McCray, J. Zabner, and A. Cong, "Practical reconstruction method for bioluminescence tomography," Opt. Express 13, 6756-6771 (2005). [CrossRef] [PubMed]
  9. X. Gu, Q. Zhang, L. Larcom, and H. Jiang, "Three-dimensional bioluminescence tomography with model-based reconstruction," Opt. Express 12, 3996-4000 (2004). [CrossRef] [PubMed]
  10. W. Cong and G. Wang, "Boundary integral method for bioluminescence tomography," J. Biomed. Opt. 11, 020503 (2006). [CrossRef] [PubMed]
  11. M. Jiang, and G. Wang, "Image reconstruction for bioluminescence tomography," in Developments in X-Ray Tomography IV, U. Bonse, ed., Proc. SPIE 5535, 335-351 (2004). [CrossRef]
  12. N.V. Slavine, M.A. Lewis, E. Richer, P.P. Antich, "Iterative reconstruction method for light emitting sources based on the diffusion equation," Med. Phys. 33, 61-68 (2006). [CrossRef] [PubMed]
  13. G. Alexandrakis, F. R. Rannou, and A. F. Chatziioannou, "Tomographic bioluminescence imaging by use of a combined optical-PET (OPET) system: a computer simulation feasibility study," Phys. Med. Biol. 50, 4225-4241 (2005). [CrossRef] [PubMed]
  14. A. J. Chaudhari, F. Darvas, J. R. Bading, R. A. Moats, P. S. Conti, D. J. Smith, S. R. Cherry and R. M. Leahy, "Hyperspectral and multispectral bioluminescence optical tomography for small animal imaging," Phys. Med. Biol. 50, 5421-5441 (2005). [CrossRef] [PubMed]
  15. A. X. Cong and G. Wang, "Multi-spectral bioluminescence tomography: Methodology and simulation," International Journal of Biomedical Imaging 2006, ID57614, 1-7 (2006).
  16. H. Dehghani, S. C. Davis, S. Jiang, B. W. Pogue, and K. D. Paulsen, "Spectrally resolved bioluminescence optical tomography," Opt. Lett. 31, 365-367 (2006). [CrossRef] [PubMed]
  17. M. Schweiger, S. R. Arridge, M. Hiraoka, and D. T. Delpy, "The finite element method for the propagation of light in scattering media: Boundary and source conditions," Med. Phys. 22, 1779- 1792 (1995). [CrossRef] [PubMed]
  18. A. J. Welch and M. J. C. Van Gemert, Optical and Thermal response of laser-irradiated tissue (Plenum Press, New York, 1995).
  19. J. M. Drake, C. L. Gabriel, and M. D. Henry, "Assessing tumor growth and distribution in a model of prostrate cancer metastasis using bioluminescence imaging," Clin. Exp. Metastasis PMID: 16703413, [Epub ahead of print] (2006). [PubMed]
  20. A. Rehemtulla, L. D. Stegman, S. J. Cardozo, S. Gupta, D. E. Hall, C. H. Contag, and B. D. Ross, "Rapid and quantitative assessment of cancer treatment response using in vivo bioluminescence imaging," Neoplasia 2, 491-495 (2000). [CrossRef]

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