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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 17 — Aug. 21, 2006
  • pp: 7841–7851

Pulsation-resolved deep tissue dynamics measured with diffusing-wave spectroscopy

Jun Li, Franck Jaillon, Gregor Dietsche, Georg Maret, and Thomas Gisler  »View Author Affiliations

Optics Express, Vol. 14, Issue 17, pp. 7841-7851 (2006)

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We present a technique for measuring transient microscopic dynamics within deep tissue with sub-second temporal resolution, using diffusing-wave spectroscopy with gated single-photon avalanche photodiodes (APDs) combined with standard ungated multi-tau correlators. Using the temporal autocorrelation function of a reference signal allows to correct the temporal intensity autocorrelation function of the sample signal for the distortions induced by the non-constant average photon count rate. We apply this technique to pulsation-synchronized measurements of tissue dynamics in humans. Measurements on the forearm show no dependence on the pulsation phase. In contrast, the decay rate of the DWS signal measured on the wrist over the radial artery shows a pulsation-induced modulation of 60–90% consistent with pulsatile variations of arterial erythrocyte flow velocity. This might make time-resolved DWS interesting as a sensitive and fast method for investigating deep tissue perfusion, e.g. in intensive care.

© 2006 Optical Society of America

OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.5280) Medical optics and biotechnology : Photon migration
(290.1350) Scattering : Backscattering
(290.1990) Scattering : Diffusion
(290.4210) Scattering : Multiple scattering

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 24, 2006
Revised Manuscript: June 13, 2006
Manuscript Accepted: July 14, 2006
Published: August 21, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Jun Li, Franck Jaillon, Gregor Dietsche, Georg Maret, and Thomas Gisler, "Pulsation-resolved deep tissue dynamics measured with diffusing-wave spectroscopy," Opt. Express 14, 7841-7851 (2006)

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