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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 18 — Sep. 4, 2006
  • pp: 8263–8268

Diffraction phase and fluorescence microscopy

YongKeun Park, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations

Optics Express, Vol. 14, Issue 18, pp. 8263-8268 (2006)

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We have developed diffraction phase and fluorescence (DPF) microscopy as a new technique for simultaneous quantitative phase imaging and epi-fluorescence investigation of live cells. The DPF instrument consists of an interference microscope, which is incorporated into a conventional inverted fluorescence microscope. The quantitative phase images are characterized by sub-nanometer optical path-length stability over periods from milliseconds to a cell lifetime. The potential of the technique for quantifying rapid nanoscale motions in live cells is demonstrated by experiments on red blood cells, while the composite phase-fluorescence imaging mode is exemplified with mitotic kidney cells.

© 2006 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.0180) Microscopy : Microscopy

ToC Category:

Original Manuscript: May 31, 2006
Revised Manuscript: August 24, 2006
Manuscript Accepted: August 25, 2006
Published: September 1, 2006

Virtual Issues
Vol. 1, Iss. 10 Virtual Journal for Biomedical Optics

YongKeun Park, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Diffraction phase and fluorescence microscopy," Opt. Express 14, 8263-8268 (2006)

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