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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 19 — Sep. 18, 2006
  • pp: 8722–8727

A single-shot approach for measuring two-photon action cross-section of fluorescent markers

Kebin Shi, Ahmed A. Heikal, and Zhiwen Liu  »View Author Affiliations

Optics Express, Vol. 14, Issue 19, pp. 8722-8727 (2006)

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We report on a novel method designed for measuring two-photon action cross sections spectra in a single shot without tuning the excitation wavelength. Our technique is based on (i) using a nonlinear photonic crystal fiber to broaden the spectrum of the femtosecond excitation pulses and (ii) exploiting angular dispersion to focus different wavelengths to different lateral positions. As a result, two-photon fluorescence signal at different excitation wavelengths can be obtained simultaneously. As a proof of principle, the relative two-photon action cross sections of rhodamine green and DiI-C18 are measured over 740–860 nm range using fluorescein as a reference. Our results are in good agreement with that obtained using conventional tunable mode-locked laser.

© 2006 Optical Society of America

OCIS Codes
(060.4370) Fiber optics and optical communications : Nonlinear optics, fibers
(180.2520) Microscopy : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Nonlinear Optics

Original Manuscript: July 25, 2006
Revised Manuscript: August 30, 2006
Manuscript Accepted: August 31, 2006
Published: September 18, 2006

Virtual Issues
Vol. 1, Iss. 10 Virtual Journal for Biomedical Optics

Kebin Shi, Ahmed A. Heikal, and Zhiwen Liu, "A single-shot approach for measuring two-photon action cross-section of fluorescent markers," Opt. Express 14, 8722-8727 (2006)

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