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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 19 — Sep. 18, 2006
  • pp: 8722–8727

A single-shot approach for measuring two-photon action cross-section of fluorescent markers

Kebin Shi, Ahmed A. Heikal, and Zhiwen Liu  »View Author Affiliations


Optics Express, Vol. 14, Issue 19, pp. 8722-8727 (2006)
http://dx.doi.org/10.1364/OE.14.008722


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Abstract

We report on a novel method designed for measuring two-photon action cross sections spectra in a single shot without tuning the excitation wavelength. Our technique is based on (i) using a nonlinear photonic crystal fiber to broaden the spectrum of the femtosecond excitation pulses and (ii) exploiting angular dispersion to focus different wavelengths to different lateral positions. As a result, two-photon fluorescence signal at different excitation wavelengths can be obtained simultaneously. As a proof of principle, the relative two-photon action cross sections of rhodamine green and DiI-C18 are measured over 740–860 nm range using fluorescein as a reference. Our results are in good agreement with that obtained using conventional tunable mode-locked laser.

© 2006 Optical Society of America

OCIS Codes
(060.4370) Fiber optics and optical communications : Nonlinear optics, fibers
(180.2520) Microscopy : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Nonlinear Optics

History
Original Manuscript: July 25, 2006
Revised Manuscript: August 30, 2006
Manuscript Accepted: August 31, 2006
Published: September 18, 2006

Virtual Issues
Vol. 1, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Kebin Shi, Ahmed A. Heikal, and Zhiwen Liu, "A single-shot approach for measuring two-photon action cross-section of fluorescent markers," Opt. Express 14, 8722-8727 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-19-8722


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References

  1. W. Denk, J. H. Strickler, and W. W. Webb, "2-Photon Laser Scanning Fluorescence Microscopy," Science 248, 73-76 (1990). [CrossRef] [PubMed]
  2. K. Konig, "Multiphoton microscopy in life sciences," J. of Microsc. 200, 83-104 (2000). [CrossRef]
  3. C. Xu and W.W. Webb, "Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm," J. Opt. Soc. Am. B 13, 481- 491 (1996). [CrossRef]
  4. R. Kappoor, C. S. Friend, and A. Patra, "Two-photon-excited absolute emission cross-sectional measurements calibrated with a luminance meter," J. Opt. Soc. Am. B 20, 1550-1554 (2003). [CrossRef]
  5. P. Kaatz, and D. S. Shelton, "Two-photon fluorescence cross-section measurements calibrated with hyper-Rayleigh scattering," J. Opt. Soc. Am. B 16, 998-1006 (1999). [CrossRef]
  6. M. Schneider, S. Barozzi, I. Testa, M. Faretta, and A. Diaspro, "Two-Photon activation and excitation properties of PA-GFP in the 720-920-nm region," Biophys. J. 89, 1346-1352 (2005). [CrossRef] [PubMed]
  7. M. A. Albota, C. Xu, and W. W. Webb, "Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm," Appl. Opt. 37, 7352-7356 (1998). [CrossRef]
  8. G. A. Blab et al., "Two-photon excitation action cross-sections of the autofluorescent proteins," Chem. Phys. Lett. 350, 71-77 (2001). [CrossRef]
  9. J. C. Knight, T. A. Birks, P. S Russell and D. M. Atkin, "All-silica single-mode optical fiber with photonic crystal cladding," Opt. Lett. 21, 1547-1549 (1996). [CrossRef] [PubMed]
  10. G. J. Tearney, R. H. Webb, and B. E. Bouma, "Spectrally encoded confocal microscopy," Opt. Lett. 23, 1152-1154 (1998). [CrossRef]
  11. K. Shi, P. Li, S. Yin, and Z. Liu, "Chromatic confocal microscopy using supercontinuum light," Opt. Express 12, 2096-2101 (2004). [CrossRef] [PubMed]
  12. K. Shi, S. Yin, and Z. Liu, "Wavelength division scanning for two-photon excitation fluorescence imaging," J. Microsc. 223, 83-87 (2006). [CrossRef] [PubMed]
  13. G. P. Agrawal, Nonlinear Fiber Optics, 2nd ed. (Academic Press, London, 1995).
  14. C. Tung et al., "Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs," in Multiphoton Microscopy in the Biomedical Sciences III, A. Periasamy, and P. T. C. So, eds., Proc. SPIE 4963, 95-104 (2003). [CrossRef]
  15. E. L. Elson, and D. Magde, "Fluorescence correlation spectroscopy. 1. Conceptual basis and theory," Biopolymers 13, 1-27 (1974). [CrossRef]
  16. J. K. Ranka, R. S. Windeler, and A. J. Stentz, "Visible continuum generation in air-silica microstructure optical fibers with anomalous dispersion at 800 nm," Opt. Lett. 25, 25-27 (2000). [CrossRef]
  17. D. Meshulach, and Y. Silberberg, "Coherent quantum control of two-photon transitions by a femtosecond laser pulse," Nature 396, 239-242 (1998). [CrossRef]
  18. J. P. Ogilvie, "Use of coherent control for selective two-photon fluorescence microscopy in live organisms," Opt. Express 14, 759-766 (2006). [CrossRef] [PubMed]

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