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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 19 — Sep. 18, 2006
  • pp: 8849–8865

Rayleigh-Debye-Gans as a model for continuous monitoring of biological particles: Part I, assessment of theoretical limits and approximations

Alicia C. Garcia-Lopez, Arthur David Snider, and Luis H. Garcia-Rubio  »View Author Affiliations

Optics Express, Vol. 14, Issue 19, pp. 8849-8865 (2006)

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A rapid tool for the characterization of submicron particles is light spectroscopy. Rayleigh-Debye-Gans and Mie theories provide light scattering solutions that can be evaluated within the time constants required for continuous real time monitoring applications, as in characterization of biological particles. A multiwavelength assessment of Rayleigh-Debye-Gans theory for spheres was conducted over the UV-Vis wavelength range where strict adherence to the limits of the theory at a single wavelength could not be met. Reported corrections to the refractive indices were developed to extend the range of application of the Rayleigh-Debye-Gans approximation. The results of this study show that there is considerable disagreement between Rayleigh-Debye-Gans and Mie theory across the UV-Vis spectrum.

© 2006 Optical Society of America

OCIS Codes
(190.0190) Nonlinear optics : Nonlinear optics
(350.4990) Other areas of optics : Particles

ToC Category:

Original Manuscript: May 25, 2006
Revised Manuscript: July 28, 2006
Manuscript Accepted: July 29, 2006
Published: September 18, 2006

Virtual Issues
Vol. 1, Iss. 10 Virtual Journal for Biomedical Optics

Alicia C. Garcia-Lopez, Arthur D. Snider, and Luis H. Garcia-Rubio, "Rayleigh-Debye-Gans as a model for continuous monitoring of biological particles: Part I, assessment of theoretical limits and approximations," Opt. Express 14, 8849-8865 (2006)

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  1. C. E. Alupoaei, J. A. Olivares, and L. H. Garcia-Rubio, "Quantitative spectroscopy analysis of prokaryotic cells: vegetative cells and spores," Biosens. Bioelectron. 19,893-903 (2003). [CrossRef]
  2. A. Katz, A. Alimova, M. Xu, E. Rudolf, M. K. Shah, H. E. Savage, R. B. Rosen, S. A. McCormick, and R. R. Alfano, "Bacteria size determination by elastic light scattering," IEEE J. Sel. Top. Quantum Electron. 9, 277-287 (2003). [CrossRef]
  3. M. I. Mishchenko, L. D. Travis, and D. W. Mackowski, "T-Matrix Computations of Light Scattering by Nonspherical Particles: A Review," J. Quant. Spectrosc. Radiat. Transf. 55, 535-575 (1996).
  4. A. L. Koch, B. R. Robertson, and D. K. Button, "Deduction of cell volume and mass from forward scatter intensity from bacteria analyzed by flow cytometry," J. Microbiol. Methods. 2, 40-61 (1996).
  5. C. F. Bohren, and D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley Science Paper Series, New York, 1998). [CrossRef]
  6. M. Kerker, The Scattering of Light and other Electromagnetic Radiation (Academic Press, New York, 1969).
  7. M. Hammer, D. Schweitzerk, B. Michel, E. Thamm, and A. Kolb, " Single scattering by red blood cells," Appl. Opt. 37, 7410-7418 (1998). [CrossRef]
  8. A. Katz, A. Alimova, M. Xu, E. Rudolf, M. K. Shah, H. E. Savage, R. B. Rosen, S. A. McCormick, and R. R. Alfano, "In situ identification of bacteria size by light scattering," Proc. SPIE 4965, 7376 (2003)
  9. B. P. Latimer, "Scattering by Ellipsoids of Revolution; A Comparison of Theoretical Methods," J. Colloid Interface Science 63, 310-316 (1977). [CrossRef]
  10. N. L. Veshkin, "Screening Hypochromism of Biological Macromolecules and Suspensions," J. Photochem. Photobiol. B,  3, 625-630 (1989). [CrossRef]
  11. N. L. Veshkin, "Screening Hypochromism of Molecular Aggregates and Biopolymers," J. Biol. Phys. 25, 339-354 (1999). [CrossRef]
  12. N. L. Veshkin, "Screening Hypochromism of Chromophores in Macromolecular Biostructures," Biophys. J. 44, 41-51 (1999)
  13. P. J. Wyatt, and D. T. Phillips, "Structure of single bacteria from light scattering," J. Theor. Biol. 37, 493-501 (1972). [CrossRef]
  14. L. H. Garcia-Rubio, Private Communication.
  15. W. J. Wiscombe, "Mie Scattering Calculations: Advances in Technique and Fast, Vector-Speed Computer Codes," NCAR/TN-140 + STR. National Center for Atmospheric Research, Boulder Colorado (1979).
  16. A. C. Garcia-LopezHybrid Model for Characterization of Submicron Particles using Mulitwavelength Spectroscopy, (University of South Florida, 2005).
  17. P. Latimer, A. Brunsting, B. E. Pyle and C. Moor, "Effects of asphericity on single particle scattering," Appl. Opt. 17, 3152-3158 (1978). [CrossRef] [PubMed]
  18. A. Nonoyama, Using multiwavelength UV-Visible spectroscopy for the characterization of red blood cells: an investigation of hypochromism. (University of South Florida, 2004).
  19. S. Narayanan, Aggregation and Structural Changes in Biological Systems: An Ultraviolet Visible Spectroscopic Approach for Analysis of Blood Cell Aggregation and Protein Conformation. (University of South Florida, 1999). [PubMed]

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