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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 2 — Jan. 23, 2006
  • pp: 717–725

Slow Ca2+ wave stimulation using low repetition rate femtosecond pulsed irradiation

S. Iwanaga, N. I. Smith, K. Fujita, and S. Kawata  »View Author Affiliations

Optics Express, Vol. 14, Issue 2, pp. 717-725 (2006)

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We demonstrated stimulation of Ca2+ in living cells by near-infrared laser pulses operated at sub-MHz repetition rates. HeLa cells were exposed to focused 780 nm femtosecond pulses, generated by a titanium-sapphire laser and adjusted by an electro-optical modulator. We found that the laser-induced Ca2+ waves could be generated over three orders of magnitude in repetition rates, with required laser pulse energy varying by less than one order of magnitude. Ca2+ wave speed and gradients were reduced with repetition rate, which allows the technique to be used to modulate the strength and speed of laser-induced effects. By lowering the repetition rate, we found that the laser-induced Ca2+ release is partially mediated by reactive oxygen species (ROS). Inhibition of ROS was successful only at low repetition rates, with the implication that ROS scavengers may in general be depleted in experiments using high repetition rate laser irradiation.

© 2006 Optical Society of America

OCIS Codes
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.2520) Microscopy : Fluorescence microscopy
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(320.7110) Ultrafast optics : Ultrafast nonlinear optics

ToC Category:
Medical Optics and Biotechnology

Virtual Issues
Vol. 1, Iss. 2 Virtual Journal for Biomedical Optics

S. Iwanaga, N. I. Smith, K. Fujita, and S. Kawata, "Slow Ca2+ wave stimulation using low repetition rate femtosecond pulsed irradiation," Opt. Express 14, 717-725 (2006)

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