OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 2 — Jan. 23, 2006
  • pp: 717–725

Slow Ca2+ wave stimulation using low repetition rate femtosecond pulsed irradiation

S. Iwanaga, N. I. Smith, K. Fujita, and S. Kawata  »View Author Affiliations


Optics Express, Vol. 14, Issue 2, pp. 717-725 (2006)
http://dx.doi.org/10.1364/OPEX.14.000717


View Full Text Article

Enhanced HTML    Acrobat PDF (439 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We demonstrated stimulation of Ca2+ in living cells by near-infrared laser pulses operated at sub-MHz repetition rates. HeLa cells were exposed to focused 780 nm femtosecond pulses, generated by a titanium-sapphire laser and adjusted by an electro-optical modulator. We found that the laser-induced Ca2+ waves could be generated over three orders of magnitude in repetition rates, with required laser pulse energy varying by less than one order of magnitude. Ca2+ wave speed and gradients were reduced with repetition rate, which allows the technique to be used to modulate the strength and speed of laser-induced effects. By lowering the repetition rate, we found that the laser-induced Ca2+ release is partially mediated by reactive oxygen species (ROS). Inhibition of ROS was successful only at low repetition rates, with the implication that ROS scavengers may in general be depleted in experiments using high repetition rate laser irradiation.

© 2006 Optical Society of America

OCIS Codes
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.2520) Microscopy : Fluorescence microscopy
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(320.7110) Ultrafast optics : Ultrafast nonlinear optics

ToC Category:
Medical Optics and Biotechnology

Virtual Issues
Vol. 1, Iss. 2 Virtual Journal for Biomedical Optics

Citation
S. Iwanaga, N. I. Smith, K. Fujita, and S. Kawata, "Slow Ca2+ wave stimulation using low repetition rate femtosecond pulsed irradiation," Opt. Express 14, 717-725 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-2-717


Sort:  Journal  |  Reset  

References

  1. K. König, I. Riemann, P. Fischer, and K.J. Halbhuber, "Intracellular nanosurgery with near infrared femtosecond laser pulses," Cell Mol. Biol. 45, 195-201 (1999). [PubMed]
  2. K. König, I. Riemann, and W. Fritzsche, "Nanodissection of human chromosomes with near-infrared femtosecond laser pulses," Opt. Lett. 26, 819-821 (2001). [CrossRef]
  3. A.B. Uzdensky, and V.V. Savransky, "Single neuron response to pulse-periodic laser microirradiation. Action spectra and two-photon effect," J. Photochem. Photobiol. B Biology 39, 224-228 (1997). [CrossRef]
  4. N. I. Smith, K. Fujita, T. Kaneko, K. Kato, O. Nakamura, T. Takamatsu, and S. Kawata, "Generation of calcium waves in living cells by pulsed-laser-induced photodisruption," Appl. Phys. Lett. 79, 1208-1210 (2001). [CrossRef]
  5. H. Hirase, V. Nikolenko, J.H. Goldberg, and R. Yuste, "Multiphoton stimulation of neurons," J. Neurobiol. 51, 237-247 (2002). [CrossRef] [PubMed]
  6. M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, "Functional regeneration after laser axotomy," Nature 432, 822 (2004). [CrossRef] [PubMed]
  7. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73-76 (1990). [CrossRef] [PubMed]
  8. S. Iwanaga, N. Smith, K. Fujita, T. Kaneko, O. Nakamura, S. Kawata, M. Oyamada, and T. Takamatsu, "Stimulation of living cells by femtosecond near-infrared laser pulses," in Commercial and Biomedical Applications of Ultrafast Lasers 3, J. Neev, A. Ostendorf, and C. B. Schaffer, eds., Proc. SPIE 4978, 122-128 (2003). [CrossRef]
  9. M. Endo, M. Tanaka, and Y. Ogawa, "Calcium induced release of calcium from the sarcoplasmic reticulum of skinned skeletal muscle fibres," Nature 228, 34-36 (1970). [CrossRef] [PubMed]
  10. M. J. Berridge "Elementary and global aspects of calcium signalling," J. Physiol. 499, 291-306 (1997). [PubMed]
  11. M. J. Berridge, P. Lipp, and M. D. Bootman, "Calcium - a life and death signal," Nature 395, 645-648 (1998). [CrossRef] [PubMed]
  12. T. Kaneko, H. Tanaka, M. Oyamada, S. Kawata, and T. Takamatsu, "Three distinct types of Ca2+ waves in Langendorff-perfused rat heart revealed by real-time confocal microscopy," Circ. Res. 86, 1093-1099 (2000). [PubMed]
  13. D. N. Wheatley, A. Redfern, and R. P. C. Johnson, "Heat-induced disturbances of intracellular movement and the consistency of the aqueous cytoplasm in HeLa S-3 cells: A laser-doppler and proton NMR study," Physiol. Chem. Phys. & Med. NMR 23, 199-216 (1991) [PubMed]
  14. S. H. Kang, and Q. Chae, "Ultraviolet Light-induced Lipid Peroxidation of Cultured Skin Fibroblast Membrane," Bull. Korean Chem. Soc., 14, 371-374 (1993).
  15. T. T. Rohn, T. R. Hinds, and F. F. Vincenzi, "Inhibition of Ca2+-pump ATPase and the Na+/K+-pump ATPase by iron-generated free radicals. Protection by 6,7-dimethyl-2,4-DI-1-pyrrolidinyl-7H-pyrrolo[2,3-d] pyrimidine sulfate (U-89843D), a potent, novel, antioxidant/free radical scavenger," Biochem. Pharmacol. 51, 471-476 (1996). [CrossRef] [PubMed]
  16. Q. Hu, S. Corda, J. L. Zweier, M. C. Capogrossi, and R. C. Ziegelstein, "Hydrogen peroxide induces intracellular calcium oscillations in human aortic endothelial cells," Circulation. 97, 268-275 (1998). [PubMed]
  17. P. E. Hockberger, T. A. Skimina, V. E. Centonze, C. Lavin, S. Chu, S. Dadras, J. K. Reddy, and J. G. White, "Activation of flavin-containing oxidases underlies light-induced production of H2O2 in mammalian cells," Proc. Natl. Acad. Sci. USA 96, 6255-6260 (1999). [CrossRef] [PubMed]
  18. U. K. Tirlapur, K. König, C. Peuckert, R. Krieg, and K.-J. Halbhuber, "Femtosecond near-infrared laser pulses elicit generation of reactive oxygen species in mammalian cells leading to apoptosis-like death," Exp. Cell. Res. 263, 88-97 (2001). [CrossRef] [PubMed]
  19. A. Mahns, I. Melchheier, C. V. Suschek, H. Sies, and Lars-Oliver Klotz, "Irradiation of cells with ultraviolet-A (320-400nm) in the presence of cell culture medium elicits biological effects due to extracellular generation of hydrogen peroxide," Free Radic. Res. 37, 391-397 (2003). [CrossRef] [PubMed]
  20. M. A. J. Rodgers, and P. T. Snowden, "Lifetime of O2 (1Δg) in liquid water as determined by time-resolved infrared luminescence measurements," J. Am. Chem. Soc. 104, 5541-5543 (1982). [CrossRef]
  21. M. S. Patterson, "Experimental tests of the feasibility of singlet oxygen luminescence monitoring in vivo during photodynamic therapy," J. Photochem. Photobiol. B 5, 69-84 (1990). [CrossRef] [PubMed]
  22. C. Batandier, E. Fontaine, C. Kériel, X. M. Leverve, "Determination of mitochondrial reactive oxygen species: methodological aspects," J. Cell Mol. Med. 6, 175-187 (2002). [CrossRef] [PubMed]
  23. L. V. Keldysh, "Ionization in the field of a strong electromagnetic wave," Sov. Phys. JETP 20, 1307-1314 (1965).
  24. A. Vogel and V. Venugopalan, "Mechanisms of pulsed laser ablation of biological tissues," Chem. Rev. 103, 577-644 (2003). [CrossRef] [PubMed]
  25. J. I. Kourie, "Interaction of reactive oxygen species with ion transport mechanisms," Am. J. Physiol. Cell Physiol. 275, C1-C24 (1998).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited