Confocal and nonlinear optical microscopies have been applied for dermatological studies because of their capability to provide sub-surface three-dimensional images with sub-µm spatial resolutions. Optical signal degradation as the imaging plane being moved toward deeper regions in skin specimens is the key factor that limits the observation depth for the laser scanning based linear or nonlinear imaging modalities. In this article, we studied the signal degradation in fixed human skin specimens using reflection confocal microscopy and higher-harmonic optical microscopy based on a Cr:forsterite femtosecond laser centered at 1230-nm. By analyzing the optical properties through these linear and nonlinear imaging modalities, we found that the optical signal degradation in the studied human skin specimen is dominated by the distortion of the point spread function.
© 2006 Optical Society of America
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.1870) Medical optics and biotechnology : Dermatology
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
Medical Optics and Biotechnology
Original Manuscript: December 9, 2005
Revised Manuscript: January 17, 2006
Manuscript Accepted: January 18, 2006
Published: January 23, 2006
Vol. 1, Iss. 2 Virtual Journal for Biomedical Optics
, "Optical signal degradation study in fixed human skin using confocal microscopy and higher-harmonic optical microscopy," Opt. Express 14, 749-758 (2006)
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