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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 20 — Oct. 2, 2006
  • pp: 9307–9316

Enhanced live cell membrane imaging using surface plasmon-enhanced total internal reflection fluorescence microscopy

Ruei-Yu He, Guan-Liang Chang, Hua-Lin Wu, Chi-Hung Lin, Kuo-Chih Chiu, Yuan-Deng Su, and Shean-Jen Chen  »View Author Affiliations

Optics Express, Vol. 14, Issue 20, pp. 9307-9316 (2006)

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Using a total internal reflection fluorescence microscopy (TIRFM) technique to image live cells on a biosurface not only provides an enhanced understanding of cellular functions, but also improves the signal-to-noise ratio of the images. However, the intensity of the fluorescence signal must be increased if a more dynamic biomolecular imaging capability is required. Accordingly, this study presents a surface plasmon-enhanced TIRFM technique in which the fluorescence signals are enhanced via surface plasmons offered by a silver nanolayer. The developed microscopy technique is successfully applied to the real-time observation of the thrombomodulin proteins of live cell membranes. The experimental results and the simulation results demonstrate that the live cell membrane images obtained in the proposed surface plasmon-enhanced TIRFM technique are brighter by approximately one order of magnitude than those provided by conventional TIRFM.

© 2006 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 26, 2006
Revised Manuscript: September 7, 2006
Manuscript Accepted: September 13, 2006
Published: October 2, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Ruei-Yu He, Guan-Liang Chang, Hua-Lin Wu, Chi-Hung Lin, Kuo-Chih Chiu, Yuan-Deng Su, and Shean-Jen Chen, "Enhanced live cell membrane imaging using surface plasmon-enhanced total internal reflection fluorescence microscopy," Opt. Express 14, 9307-9316 (2006)

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