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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 20 — Oct. 2, 2006
  • pp: 9317–9323

In vivo volumetric imaging of subcutaneous microvasculature by photoacoustic microscopy

Hao F. Zhang, Konstantin Maslov, Meng-Lin Li, George Stoica, and Lihong V. Wang  »View Author Affiliations

Optics Express, Vol. 14, Issue 20, pp. 9317-9323 (2006)

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Photoacoustic microscopy was developed to achieve volumetric imaging of the anatomy and functions of the subcutaneous microvasculature in both small animals and humans in vivo with high spatial resolution and high signal-to-background ratio. By following the skin contour in raster scanning, the ultrasonic transducer maintains focusing in the region of interest. Furthermore, off-focus lateral resolution is improved by using a synthetic-aperture focusing technique based on the virtual point detector concept. Structural images are acquired in both rats and humans, whereas functional images representing hemoglobin oxygen saturation are acquired in rats. After multiscale vesselness filtering, arterioles and venules in the image are separated based on the imaged oxygen saturation levels. Detailed structural information, such as vessel depth and spatial orientation, are revealed by volume rendering.

© 2006 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 20, 2006
Revised Manuscript: August 14, 2006
Manuscript Accepted: August 14, 2006
Published: October 2, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Hao F. Zhang, Konstantin Maslov, Meng-Lin Li, George Stoica, and Lihong V. Wang, "In vivo volumetric imaging of subcutaneous microvasculature by photoacoustic microscopy," Opt. Express 14, 9317-9323 (2006)

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