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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 21 — Oct. 16, 2006
  • pp: 9825–9831

Two-photon microscopy with wavelength switchable fiber laser excitation

Jay R. Unruh, E. Shane Price, Roque Gagliano Molla, Lisa Stehno-Bittel, Carey K. Johnson, and Rongqing Hui  »View Author Affiliations

Optics Express, Vol. 14, Issue 21, pp. 9825-9831 (2006)

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Two-photon scanning fluorescence microscopy has become a powerful tool for imaging living cells and tissues. Most applications of two-photon microscopy employ a Ti:sapphire laser excitation source, which is not readily portable or rapidly tunable. This work explores the use of two-photon fiber laser excitation (TP-FLEX) as an excitation source for scanning two-photon microscopy. We have further demonstrated the use of a photonic crystal fiber (PCF) for facile tuning of the excitation wavelength over the range from 810 nm to 1100 nm. We generated two-photon fluorescence images at excitation wavelengths from 850 nm to 1100 nm detected on a scanning-stage microscope. By PCF wavelength tuning the dye BODIPY fl was selectively excited at 1000 nm whereas MitoTracker red was excited preferentially at 1100 nm. We discuss the potential for fiber laser sources coupled with PCF wavelength tuning as an attractive tunable excitation source for two-photon scanning fluorescence microscopy.

© 2006 Optical Society of America

OCIS Codes
(060.4370) Fiber optics and optical communications : Nonlinear optics, fibers
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy

ToC Category:

Original Manuscript: July 18, 2006
Revised Manuscript: September 27, 2006
Manuscript Accepted: September 30, 2006
Published: October 16, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Jay R. Unruh, E. S. Price, Roque G. Molla, Lisa Stehno-Bittel, Carey K. Johnson, and Rongqing Hui, "Two-photon microscopy with wavelength switchable fiber laser excitation," Opt. Express 14, 9825-9831 (2006)

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