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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 23 — Nov. 13, 2006
  • pp: 11204–11214

Highly sensitive spectral interferometric four-wave mixing microscopy near the shot noise limit and its combination with two-photon excited fluorescence microscopy

Keisuke Isobe, Yasuyuki Ozeki, Takehito Kawasumi, Shogo Kataoka, Shin’ichiro Kajiyama, Kiichi Fukui, and Kazuyoshi Itoh  »View Author Affiliations

Optics Express, Vol. 14, Issue 23, pp. 11204-11214 (2006)

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We present spectral interferometric four-wave mixing (FWM) microscopy with a nearly shot-noise limited sensitivity and with the capability of separating FWM signals from fluorescence signals. We analyze the requirements for obtaining the shot-noise limited sensitivity and experimentally achieve the sensitivity that is only 4-dB lower than the shotnoise limit. Moreover, we show that only FWM signals can be extracted through the Fourier filtering even when the FWM spectrum is overlapped and overwhelmed by the fluorescence spectrum. We demonstrate simultaneous acquisition of FWM and two-photon excited fluorescence images of fluorescent monodispersed polystyrene microspheres.

© 2006 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(190.4410) Nonlinear optics : Nonlinear optics, parametric processes
(300.1030) Spectroscopy : Absorption
(300.6290) Spectroscopy : Spectroscopy, four-wave mixing
(320.7110) Ultrafast optics : Ultrafast nonlinear optics

ToC Category:

Original Manuscript: September 19, 2006
Revised Manuscript: October 22, 2006
Manuscript Accepted: October 24, 2006
Published: November 13, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Keisuke Isobe, Yasuyuki Ozeki, Takehito Kawasumi, Shogo Kataoka, Shin'ichiro Kajiyama, Kiichi Fukui, and Kazuyoshi Itoh, "Highly sensitive spectral interferometric four-wave mixing microscopy near the shot noise limit and its combination with two-photon excited fluorescence microscopy," Opt. Express 14, 11204-11214 (2006)

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