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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 25 — Dec. 11, 2006
  • pp: 12096–12108

Three-dimensional identification of biological microorganism using integral imaging

Bahram Javidi, Inkyu Moon, and Seokwon Yeom  »View Author Affiliations

Optics Express, Vol. 14, Issue 25, pp. 12096-12108 (2006)

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In this paper, we address the identification of biological microorganisms using microscopic integral imaging (II). II senses multiview directional information of 3D objects illuminated by incoherent light. A micro-lenslet array generates a set of elemental images by projecting a 3D scene onto a detector array. In computational reconstruction of II, 3D volumetric scenes are numerically reconstructed by means of a geometrical ray projection method. The identification of the biological samples is performed using the 3D volume of the reconstructed object. In one approach, the multivariate statistical distribution of the reference sample is measured in 3D space and compared with an unknown input sample by means of statistical discriminant functions. The multivariate empirical cumulative density of the 3D volume image is determined for classification. On the other approach, the graph matching technique is applied to 3D volumetric images with Gabor feature extraction. The reference morphology is identified in unknown input samples using 3D grids. Experimental results are presented for the identification of sphacelaria alga and tribonema aequale alga. We present experimental results for both 3D and 2D imaging. To the best of our knowledge, this is the first report on 3D identification of microorganisms using II.

© 2006 Optical Society of America

OCIS Codes
(080.0080) Geometric optics : Geometric optics
(110.6880) Imaging systems : Three-dimensional image acquisition

ToC Category:
Imaging Systems

Original Manuscript: June 22, 2006
Revised Manuscript: October 2, 2006
Manuscript Accepted: October 12, 2006
Published: December 11, 2006

Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics

Bahram Javidi, Inkyu Moon, and Seokwon Yeom, "Three-dimensional identification of biological microorganism using integral imaging," Opt. Express 14, 12096-12108 (2006)

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