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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 25 — Dec. 11, 2006
  • pp: 12473–12484

Angular resolved light scattering for discriminating among marine picoplankton: modeling and experimental measurements

Bing Shao, Jules S. Jaffe, Mirianas Chachisvilis, and Sadik C. Esener  »View Author Affiliations

Optics Express, Vol. 14, Issue 25, pp. 12473-12484 (2006)

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In order to assess the capability to optically identify small marine microbes, both simulations and experiments of angular resolved light scattering (ARLS) were performed. After calibration with 30-nm vesicles characterized by a nearly constant scattering distribution for vertically polarized light (azimuthal angle=90°), ARLS from suspensions of three types of marine picoplankton (two prokaryotes and one eukaryote) in seawater was measured with a scattering device that consisted of an elliptical mirror, a rotating aperture, and a PMT. Scattered light was recorded with adequate signal-to-noise in the 40–140°. Simulations modeled the cells as prolate spheroids with independently measured dimensions. For the prokaryotes, approximated as homogeneous spheroids, simulations were performed using the RM (Rayleigh-Mie) – I method, a hybrid of the Rayleigh-Debye approximation and the generalized Lorentz-Mie theory. For the picoeukaryote, an extended RM – I method was developed for a coated spheroid with different shell thickness distributions. The picoeukaryote was then modeled as a coated sphere with a spherical core. Good overall agreements were obtained between simulations and experiments. The distinctive scattering patterns of the different species hold promise for an identification system based on ARLS.

© 2006 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(290.4020) Scattering : Mie theory
(290.5820) Scattering : Scattering measurements
(290.5850) Scattering : Scattering, particles

ToC Category:

Original Manuscript: September 12, 2006
Revised Manuscript: November 28, 2006
Manuscript Accepted: November 29, 2006
Published: December 11, 2006

Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics

Bing Shao, Jules S. Jaffe, Mirianas Chachisvilis, and Sadik C. Esener, "Angular resolved light scattering for discriminating among marine picoplankton: modeling and experimental measurements," Opt. Express 14, 12473-12484 (2006)

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