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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 3 — Feb. 6, 2006
  • pp: 1175–1181

Three-dimensional image formation in fiber-optical second-harmonic-generation microscopy

Min Gu and Ling Fu  »View Author Affiliations


Optics Express, Vol. 14, Issue 3, pp. 1175-1181 (2006)
http://dx.doi.org/10.1364/OE.14.001175


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Abstract

Three-dimensional (3-D) image formation in fiber-optical second-harmonic-generation microscopy is revealed to be purely coherent and therefore can be described by a 3-D coherent transfer function (CTF) that exhibits the same spatial frequency passband as that of fiber-optical reflection-mode non-fluorescence microscopy. When the numerical aperture of the fiber is much larger than the angle of convergence of the illumination on the fiber aperture, the performance of fiber-optical second-harmonic-generation microscopy behaves as confocal second-harmonic-generation microscopy. The dependence of axial resolution on fiber coupling parameters shows an improvement of approximately 7%, compared with that in fiber-optical two-photon fluorescence microscopy.

© 2006 Optical Society of America

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(110.2990) Imaging systems : Image formation theory
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 28, 2005
Revised Manuscript: January 23, 2006
Manuscript Accepted: January 23, 2006
Published: February 6, 2006

Virtual Issues
Vol. 1, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Min Gu and Ling Fu, "Three-dimensional image formation in fiber-optical second-harmonic-generation microscopy," Opt. Express 14, 1175-1181 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-3-1175


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