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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 7 — Apr. 3, 2006
  • pp: 2650–2656

Programmable vector point-spread function engineering

Michael R. Beversluis, Lukas Novotny, and Stephan J. Stranick  »View Author Affiliations

Optics Express, Vol. 14, Issue 7, pp. 2650-2656 (2006)

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We use two nematic liquid crystal spatial light modulators (SLM’s) to control the vector point spread function (VPSF) of a 1.3 numerical aperture (NA) microscope objective. This is achieved by controlling the polarization and relative phase of the electric field in the objective’s pupil. We measure the resulting VPSF’s for several different pupil field polarization states. By using single fluorescent molecules as local field probes, we are able to map out the focal field distributions and polarization purity of the synthesized fields. We report the achieved field purity and address the experimental issues that currently limit it.

© 2006 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(230.6120) Optical devices : Spatial light modulators

ToC Category:
Imaging Systems

Original Manuscript: February 16, 2006
Revised Manuscript: March 27, 2006
Manuscript Accepted: March 28, 2006
Published: April 3, 2006

Virtual Issues
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics

Michael R. Beversluis, Lukas Novotny, and Stephan J. Stranick, "Programmable vector point-spread function engineering," Opt. Express 14, 2650-2656 (2006)

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