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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 8 — Apr. 17, 2006
  • pp: 3467–3471

Two-photon bioimaging with picosecond optical pulses from a semiconductor laser

Hiroyuki Yokoyama, Hengchang Guo, Takuya Yoda, Keijiro Takashima, Ki-ichi Sato, Hirokazu Taniguchi, and Hiromasa Ito  »View Author Affiliations

Optics Express, Vol. 14, Issue 8, pp. 3467-3471 (2006)

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Toward a practical light source for two-photon bioimaging, we have generated kilowatt peak power of 0.77 μm wavelength and 5 ps optical pulse via second-harmonic generation of the amplified output from a gain-switched 1.55 μm semiconductor laser. This compact scheme and stable optical-pulse-source has been successfully used for the two-photon fluorescence bioimaging of actin filaments in PtK2 cells.

© 2006 Optical Society of America

OCIS Codes
(140.5960) Lasers and laser optics : Semiconductor lasers
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Nonlinear Optics

Original Manuscript: February 17, 2006
Revised Manuscript: March 31, 2006
Manuscript Accepted: March 31, 2006
Published: April 17, 2006

Virtual Issues
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics

Hiroyuki Yokoyama, Hengchang Guo, Takuya Yoda, Keijiro Takashima, Ki-ichi Sato, Hirokazu Taniguchi, and Hiromasa Ito, "Two-photon bioimaging with picosecond optical pulses from a semiconductor laser," Opt. Express 14, 3467-3471 (2006)

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