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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 9 — May. 1, 2006
  • pp: 3792–3805

Quantitative imaging of complex samples by spiral phase contrast microscopy

Stefan Bernet, Alexander Jesacher, Severin Fürhapter, Christian Maurer, and Monika Ritsch-Marte  »View Author Affiliations


Optics Express, Vol. 14, Issue 9, pp. 3792-3805 (2006)
http://dx.doi.org/10.1364/OE.14.003792


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Abstract

Recently a spatial spiral phase filter in a Fourier plane of a microscopic imaging setup has been demonstrated to produce edge enhancement and relief-like shadow formation of amplitude and phase samples. Here we demonstrate that a sequence of at least 3 spatially filtered images, which are recorded with different rotational orientations of the spiral phase plate, can be used to obtain a quantitative reconstruction of both, amplitude and phase information of a complex microscopic sample, i.e. an object consisting of mixed absorptive and refractive components. The method is demonstrated using a calibrated phase sample, and an epithelial cheek cell.

© 2006 Optical Society of America

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(090.1970) Holography : Diffractive optics
(100.5090) Image processing : Phase-only filters

ToC Category:
Image Processing

History
Original Manuscript: March 8, 2006
Revised Manuscript: April 25, 2006
Manuscript Accepted: April 25, 2006
Published: May 1, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Stefan Bernet, Alexander Jesacher, Severin Fürhapter, Christian Maurer, and Monika Ritsch-Marte, "Quantitative imaging of complex samples by spiral phase contrast microscopy," Opt. Express 14, 3792-3805 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-9-3792


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References

  1. S. N. Khonina, V. V. Kotlyar, M. V. Shinkaryev, V. A. Soifer, and G. V. Uspleniev, "The phase rotor filter," J. Mod. Opt. 39,1147-1154 (1992). [CrossRef]
  2. Z. Jaroszewicz and A. Kolodziejczyk, "Zone plates performing generalized Hankel transforms and their metrological applications," Opt. Commun. 102, 391-396 (1993). [CrossRef]
  3. G. A. Swartzlander, Jr., "Peering into darkness with a vortex spatial filter," Opt. Lett. 26,497-499 (2001). [CrossRef]
  4. J. A. Davis, D. E. McNamara, D. M. Cottrell, and J. Campos, "Image processing with the radial Hilbert transform: theory and experiments," Opt. Lett. 25,99-101 (2000). [CrossRef]
  5. K. Crabtree, J. A. Davis, and I. Moreno, "Optical processing with vortex-producing lenses," Appl. Opt. 43,1360-1367 (2004). [CrossRef] [PubMed]
  6. S.  Fürhapter, A. Jesacher, S. Bernet, and M. Ritsch-Marte, "Spiral phase contrast imaging in microscopy," Opt. Express 13,689-694 (2005). [CrossRef] [PubMed]
  7. S. Fürhapter, A. Jesacher, S. Bernet, and M. Ritsch-Marte, "Spiral interferometry," Opt. Lett. 30,1953-1955 (2005). [CrossRef] [PubMed]
  8. A. Jesacher, S. F¨urhapter, S. Bernet, and M. Ritsch-Marte, "Spiral interferogram analysis," to appear in JOSA A (2006).
  9. A. Jesacher, S. F¨urhapter, S. Bernet, and M. Ritsch-Marte, "Shadow effects in spiral phase contrast microscopy," Phys. Rev. Lett. 94,233902 (2005). [CrossRef] [PubMed]
  10. M. R. Arnison, K. G. Larkin, C. J. R. Sheppard, N. I. Smith, and C. J. Cogswell, "Linear phase imaging using differential interference contrast microscopy," J. Microscopy 214,7-12 (2004). [CrossRef]
  11. K. G. Larkin, D. J. Bone, and M. A. Oldfield, "Natural demodulation of two-dimensional fringe patterns. I. General background of the spiral phase quadrature transform," J. Opt. Soc. Am. A,  18,1862-1870 (2001). [CrossRef]
  12. J. Villa, I. De la Rosa, G. Miramontes, and J. A. Quiroga, "Phase recovery from a single fringe pattern using an orientational vector-field-regularized estimator," J. Opt. Soc. Am. A,  22,2766-2773 (2005). [CrossRef]
  13. WeiWang, T. Yokozeki, R. Ishijima, A.Wada, Y. Miyamoto, M. Takeda, S. G. Hanson, "Optical vortex metrology for nanometric speckle displacement measurement," Opt. Express 14,120-127 (2006). [CrossRef] [PubMed]
  14. S. S. R. Oemrawsingh, J. A.W. van Houwelingen, E. R. Eliel, J. P. Woerdman, E. J. K. Verstegen, J. G. Kloosterboer, and G. W. ’t Hooft, "Production and characterization of spiral phase plates for optical wavelengths," Appl. Opt. 43,688-694 (2004). [CrossRef] [PubMed]
  15. N. R. Heckenberg, R. McDuff, C. P. Smith, and A. G. White, "Generation of optical phase singularities by computer-generated holograms," Opt. Lett. 17,221-223 (1992). [CrossRef] [PubMed]
  16. H. Kadono, M. Ogusu, and S. Toyooka, "Phase shifting common path interferometer using a liquid-crystal phase modulator," Opt. Commun. 110,391-400 (1994). [CrossRef]
  17. A. Y. M. NG, C. W. See, and M. G. Somekh, "Quantitative optical microscope with enhanced resolution using a pixelated liquid crystal spatial light modulator," J. Microscopy 214,334-340 (2004). [CrossRef]
  18. G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, "Fourier phase microscopy for investigation of biological structures and dynamics," Opt. Lett. 29,2503-2502 (2004). [CrossRef] [PubMed]
  19. K. G. Larkin, "Uniform estimation of orientation using local and nonlocal 2-D energy operators," Opt. Express 13, 8097-(8121) (2005). [CrossRef] [PubMed]
  20. G. O. Reynolds, J. B. DeVelis, G. B. Parrent, Jr., and B. J. Thompson, The new physical optics notebook: Tutorials in Fourier optics (SPIE Optical Engineering Press, Bellingham, Washington, 1989). [CrossRef]

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