High-speed autofocusing of a cell using diffraction pattern

doi:10.1364/OE.14.003952

Optics Express

Editor: Michael Duncan
Vol. 14, Iss. 9 — May. 1, 2006
pp: 3952–3960

High-speed autofocusing of a cell using diffraction pattern

Hiromasa Oku, Masatoshi Ishikawa, Theodorus , and Koichi Hashimoto »View Author Affiliations

Optics Express, Vol. 14, Issue 9, pp. 3952-3960 (2006)
http://dx.doi.org/10.1364/OE.14.003952

View Full Text Article

Enhanced HTML Acrobat PDF (1121 KB)

Journal Search
Article Lookup

Article Tools

Share

Citations

Alert me when this article is cited
Export Citation/Save

Abstract
Article Info
References (10)
Cited By
Figures (8)
Multimedia (1)
Metrics
Related Content

Abstract

This paper proposes a new autofocusing method for observing cells under a transmission illumination. The focusing method uses a quick and simple focus estimation technique termed “depth from diffraction,” which is based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed auto-focusing. To demonstrate the method, it was applied to continuous focus tracking of a swimming paramecium, in combination with two-dimensional position tracking. Three-dimensional tracking of the paramecium for 70 s was successfully demonstrated.

OCIS Codes
(100.5010) Image processing : Pattern recognition
(170.5810) Medical optics and biotechnology : Scanning microscopy

ToC Category:
Microscopy

History
Original Manuscript: February 27, 2006
Revised Manuscript: April 12, 2006
Manuscript Accepted: April 13, 2006
Published: May 1, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Hiromasa Oku, Masatoshi Ishikawa, Theodorus , and Koichi Hashimoto, "High-speed autofocusing of a cell using diffraction pattern," Opt. Express 14, 3952-3960 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-9-3952

Sort: Author | Year | Journal | Reset

References

J. H. Price and D. A. Gough, "Comparison of Phase-Contrast and Fluorescence Digital Autofocus for Scanning Microscopy," Cytometry 16, 283-297 (1994). [CrossRef]
M. Subbarao and J.-K. Tyan, "Selecting the Optimal Focus Measure for Autofocusing and Depth-From-Focus," IEEE Trans. Patternn Anal. Mach. Intell. 20, 864-870 (1998). [CrossRef]
J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Greets, " Robust Autofocusing in Microscopy," Cytometry 39, 1-9 (2000). [CrossRef] [PubMed]
M. Born and E. Wolf, Principles of Optics, 7th Edition (Cambridge University Press, Cambridge, 2002).
W. D. Nesse, Introduction to Optical Mineralogy. (Oxford University Press, New York, 1991).
T. Tsuruta. Oyo-kogaku (Applied Optics) I. (Baifukan, Tokyo, 1990). (in Japanese)
H. Toyoda, N. Mukohzaka, K. Nakamura, M. Takumi, S. Mizuno, and M. Ishikawa, "1ms column-parallel vision system coupled with an image intensifier; I-CPV," in Proceedings of Symp. High Speed Photography and Photonics 2001, vol. 5-1, 2001, pp. 89-92 (in Japanese).
Y. Nakabo, M. Ishikawa, H. Toyoda, and S. Mizuno, "1ms column parallel vision system and it’s application of high speed target tracking," in Proceedings of the IEEE International Conference on Robotics & Automation (Institute of Electrical and Electronics Engineers, New York, 2000), pp. 650-655.
H. Oku, N. Ogawa, K. Hashimoto, and M. Ishikawa, " Two-dimensional tracking of a motile microorganism allowing high-resolution observation with various imaging techniques," Rev. Sci. Instrum. 76, 034301 (2005). [CrossRef]
J. W. Goodman, Introduction to Fourier Optics. (McGraw-Hill, Inc., Boston, Massachusetts, 1996).

Cornelissen, F.
Geusebroek, J.-M.
Gough, D. A.
Greets, H.
Hashimoto, K.
Ishikawa, M.
Ogawa, N.
Oku, H.
Price, J. H.
Smeulders, A. W. M.
Subbarao, M.
Tyan, J.-K.

Cytometry

J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Greets, " Robust Autofocusing in Microscopy," Cytometry 39, 1-9 (2000). [CrossRef] [PubMed]
J. H. Price and D. A. Gough, "Comparison of Phase-Contrast and Fluorescence Digital Autofocus for Scanning Microscopy," Cytometry 16, 283-297 (1994). [CrossRef]

IEEE Trans. Patternn Anal. Mach. Intell.

M. Subbarao and J.-K. Tyan, "Selecting the Optimal Focus Measure for Autofocusing and Depth-From-Focus," IEEE Trans. Patternn Anal. Mach. Intell. 20, 864-870 (1998). [CrossRef]

Rev. Sci. Instrum.

H. Oku, N. Ogawa, K. Hashimoto, and M. Ishikawa, " Two-dimensional tracking of a motile microorganism allowing high-resolution observation with various imaging techniques," Rev. Sci. Instrum. 76, 034301 (2005). [CrossRef]

Other

J. W. Goodman, Introduction to Fourier Optics. (McGraw-Hill, Inc., Boston, Massachusetts, 1996).
M. Born and E. Wolf, Principles of Optics, 7th Edition (Cambridge University Press, Cambridge, 2002).
W. D. Nesse, Introduction to Optical Mineralogy. (Oxford University Press, New York, 1991).
T. Tsuruta. Oyo-kogaku (Applied Optics) I. (Baifukan, Tokyo, 1990). (in Japanese)
H. Toyoda, N. Mukohzaka, K. Nakamura, M. Takumi, S. Mizuno, and M. Ishikawa, "1ms column-parallel vision system coupled with an image intensifier; I-CPV," in Proceedings of Symp. High Speed Photography and Photonics 2001, vol. 5-1, 2001, pp. 89-92 (in Japanese).
Y. Nakabo, M. Ishikawa, H. Toyoda, and S. Mizuno, "1ms column parallel vision system and it’s application of high speed target tracking," in Proceedings of the IEEE International Conference on Robotics & Automation (Institute of Electrical and Electronics Engineers, New York, 2000), pp. 650-655.

2005, Oku, Rev. Sci. Instrum.
2000, Geusebroek, Cytometry
1998, Subbarao, IEEE Trans. Patternn Anal. Mach. Intell.
1994, Price, Cytometry

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Click here to see a list of articles that cite this paper