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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 1 — Jan. 8, 2007
  • pp: 76–82

Dynamics of cancer cell filopodia characterized by super-resolution bright-field optical microscopy

Tsi-Hsuan Hsu, Wei-Yu Liao, Pan-Chyr Yang, Chun-Chieh Wang, Jian-Long Xiao, and Chau-Hwang Lee  »View Author Affiliations

Optics Express, Vol. 15, Issue 1, pp. 76-82 (2007)

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We explore the dynamics of cancer cell filopodia of diameters around 200 nm by using super-resolution bright-field optical microscopy. The high contrast required by the super-resolution image-restoration process is from the nanometer topographic sensitivity of non-interferometric widefield optical profilometry, rather than fluorescence labeling. Because the image-acquisition rate of this bright-field system is 20 frames/min, fast cellular dynamics can be captured and then analyzed. We successfully observe the growth and activities of the filopodia of a CL1–0 lung cancer cell. In the culturing condition, we measure that the filopodia exhibit an average elongation rate of 90 nm/sec, and an average shrinkage rate of 75 nm/sec. With the treatment of epidermal growth factor, the elongation and shrinkage rates increase to 110 nm/sec and 100 nm/sec respectively. We also find that the treatment of epidermal growth factor raises the number of filopodia by nearly a factor of 2, which implies enhancement of cell motility.

© 2007 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Image Processing

Original Manuscript: October 31, 2006
Revised Manuscript: December 22, 2006
Manuscript Accepted: January 2, 2007
Published: January 8, 2007

Virtual Issues
Vol. 2, Iss. 2 Virtual Journal for Biomedical Optics

Tsi-Hsuan Hsu, Wei-Yu Liao, Pan-Chyr Yang, Chun-Chieh Wang, Jian-Long Xiao, and Chau-Hwang Lee, "Dynamics of cancer cell filopodia characterized by super-resolution bright-field optical microscopy," Opt. Express 15, 76-82 (2007)

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