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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 11 — May. 28, 2007
  • pp: 6696–6716

Three-dimensional in vivo fluorescence diffuse optical tomography of breast cancer in humans

Alper Corlu, Regine Choe, Turgut Durduran, Mark A. Rosen, Martin Schweiger, Simon R. Arridge, Mitchell D. Schnall, and Arjun G. Yodh  »View Author Affiliations

Optics Express, Vol. 15, Issue 11, pp. 6696-6716 (2007)

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We present three-dimensional (3D) in vivo images of human breast cancer based on fluorescence diffuse optical tomography (FDOT). To our knowledge, this work represents the first reported 3D fluorescence tomography of human breast cancer in vivo. In our protocol, the fluorophore Indocyanine Green (ICG) is injected intravenously. Fluorescence excitation and detection are accomplished in the soft-compression, parallel-plane, transmission geometry using laser sources at 786 nm and spectrally filtered CCD detection. Phantom and in vivo studies confirm the signals are due to ICG fluorescence, rather than tissue autofluorescence and excitation light leakage. Fluorescence images of breast tumors were in good agreement with those of MRI, and with DOT based on endogenous contrast. Tumor-to-normal tissue contrast based on ICG fluorescence was two-to-four-fold higher than contrast based on hemoglobin and scattering parameters. In total the measurements demonstrate that FDOT of breast cancer is feasible and promising.

© 2007 Optical Society of America

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3830) Medical optics and biotechnology : Mammography
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 5, 2007
Revised Manuscript: May 9, 2007
Manuscript Accepted: May 10, 2007
Published: May 16, 2007

Virtual Issues
Vol. 2, Iss. 6 Virtual Journal for Biomedical Optics

Alper Corlu, Regine Choe, Turgut Durduran, Mark A. Rosen, Martin Schweiger, Simon R. Arridge, Mitchell D. Schnall, and Arjun G. Yodh, "Three-dimensional in vivo fluorescence diffuse optical tomography of breast cancer in humans," Opt. Express 15, 6696-6716 (2007)

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